Polymorphism of open reading frame 71 of equine herpesvirus-4 (EHV-4) and EHV-1

Author:

Huang Jin-an1,Ficorilli Nino1,Hartley Carol A.1,Allen George P.2,Studdert Michael J.1

Affiliation:

1. Centre for Equine Virology, School of Veterinary Science, The University of Melbourne, Parkville, Victoria 3010, Australia1

2. Gluck Equine Research Centre, University of Kentucky, Lexington, Kentucky 40546, USA2

Abstract

Open reading frame (ORF) 71 genes of both equine herpesvirus-1 (EHV-1) and EHV-4 encode a unique glycoprotein, which has been described to vary in molecular mass from 200 to 450 kDa. Using PCR and nucleotide sequence analysis, it was shown that the ORF 71 genes of EHV-1 and EHV-4 are polymorphic due to a variable number of reiterated sequences in two regions, designated regions A and B. Region A was threonine-rich and was located near the N terminus. Region B comprised a 38 amino acid repeat near the C terminus that expanded following cell culture adaptation. Western blot analysis of viruses showed that EHV-4 gp2 was modified by glycosylation and that variation in region A resulted in the marked differences in the molecular mass of EHV-4 gp2.

Publisher

Microbiology Society

Subject

Virology

Reference23 articles.

1. Molecular epizootiology, pathogenesis, and prophylaxis of equine herpesvirus-1 infections;Allen;Progress in Veterinary Microbiology and Immunology,1986

2. Use of lambda gt11 and monoclonal antibodies to map the genes for the six major glycoproteins of equine herpesvirus 1;Allen;Journal of Virology,1987

3. Alterations in the equine herpesvirus 1 genome after in vitro and in vivo virus passage;Allen;Infection and Immunity,1983

4. Comparative studies of the proteins of equine herpesviruses 4 and 1 and asinine herpesvirus 3: antibody response of the natural hosts;Crabb;Journal of General Virology,1990

5. Equine herpesviruses 4 (equine rhinopneumonitis virus) and 1 (equine abortion virus);Crabb;Advances in Virus Research,1995

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