Affiliation:
1. College of Life Sciences, Zhejiang University, Hangzhou 310058, PR China
2. School of Forestry and Biotechnology, Zhejiang Agriculture & Forestry University, Linan 311300, PR China
Abstract
A Gram-stain-negative, aerobic, orange-pigmented, rod-shaped and non-motile bacterium, designated strain A6B8T, was isolated from seawater of the Mariana Trench. The isolate grew at 4–50 °C (optimum 30–35 °C), at pH 6.5–8.0 (optimum pH 7.5) and with 0.5–4.0 % (w/v) NaCl (optimum 1.0–2.0 %). Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain A6B8T was related most closely to the genus
Muriicola
and shared highest sequence similarity of 97.7 % with
Muriicola jejuensis
EM44T. Chemotaxonomic analysis showed menaquinone 6 (MK-6) was the predominant isoprenoid and iso-C15 : 0, iso-C15 : 1 G and iso-C17 : 0 3-OH were the major cellular fatty acids. The polar lipid profile of strain A6B8T included phosphatidylethanolamine, three unidentified aminolipids and four unidentified lipids. The genomic DNA G+C content was 47.1 mol%. The DNA–DNA relatedness value (23.3 %) clearly demonstrated that strains A6B8T and
M. jejuensis
EM44T were representatives of two different species. Based on the phenotypic, phylogenetic and chemotaxonomic characterizations, A6B8T ( = CGMCC 1.12606T = KCTC 32436T) is considered to be the type strain of a novel species of the genus
Muriicola
, for which the name Muriicola marianensis sp. nov. is proposed.
Funder
Open Research Program of the Key Laboratory of Marine Ecosystem and Biogeochemistry (LMEB), State Oceanic Administration
National Natural Science Foundation of China
National Basic Research Program of China
Subject
General Medicine,Ecology, Evolution, Behavior and Systematics,Microbiology
Reference25 articles.
1. Zeaxanthinibacter enoshimensis gen. nov., sp. nov., a novel zeaxanthin-producing marine bacterium of the family Flavobacteriaceae, isolated from seawater off Enoshima Island, Japan;Asker;Int J Syst Evol Microbiol,2007
2. Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family;Bernardet;Int J Syst Evol Microbiol,2002
3. Evolutionary trees from DNA sequences: a maximum likelihood approach;Felsenstein;J Mol Evol,1981
4. Toward defining course of evolution - minimum change for a specific tree topology;Fitch;Syst Zool,1971
5. Aquibacter zeaxanthinifaciens gen. nov., sp. nov., a zeaxanthin-producing bacterium of the family Flavobacteriaceae isolated from surface seawater, and emended descriptions of the genera Aestuariibaculum and Gaetbulibacter;Hameed;Int J Syst Evol Microbiol,2014
Cited by
8 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献