Microbiological and molecular screening of Candida spp. isolated from genital tract of asymptomatic pregnant women

Author:

Bouglita Wafa123,Rabhi Sameh3,Raich Natacha4,Bouabid Cyrine23,Belghith Cyrine5,Slimani Olfa5,Hkimi Chaima6,Ghedira Kais6,Karess Roger E.4,Guizani-Tabbane Lamia3,Attia Leila5,Rabhi Imen31ORCID

Affiliation:

1. Higher Institute of Biotechnology of Sidi Thabet, University of Manouba, Manouba, Tunisia

2. Université Tunis El-Manar, 13 Place Pasteur -BP74, 1002 Tunis-Belvédère, Tunisia

3. Laboratoire de Parasitologie Médicale, Biotechnologie and Biomolecules, Institut Pasteur de Tunis, Tunis, Tunisia

4. Université de Paris Cité, CNRS, Institut Jacques Monod, F-750013 Paris, France

5. Service de Gynécologie Obstétrique A, Hôpital Charles Nicolle, Faculté de Médecine de Tunis, Tunis, Tunisia

6. Laboratory of Bioinformatics, BioMathematics and Biostatistics (LR16IPT09), Pasteur Institute of Tunisia, University of Tunis, El Manar, 1002 Tunis, Tunisia

Abstract

Introduction. Candida spp. may cause opportunistic infections called vulvovaginal candidiasis (VVC), which is estimated to be the second most common cause of vaginitis worldwide. Gap Statement. Under various circumstances, VVC could compromise pregnancy outcomes. Emerging data suggests that VVC during pregnancy may be associated with increased risk of complications and congenital cutaneous candidiasis. Aim. To assess the prevalence of Candida spp. in asymptomatic pregnant women and determine the susceptibility of the isolates to antifungal drugs. Methodology. In a prospective cohort, 65 high vaginal swab samples of consented pregnant women. Candida isolates were identified using both microbiological and molecular tools and drug susceptibilities were profiled. Results. The prevalence of VVC among our study participants was 37 %, 24 of the 65 asymptomatic pregnant women show Candida spp. colonization. C. albicans was the most common species 61 %, followed by C. glabrata 39 %. In addition, a significant fraction of the isolated colonies showed resistance to Fluconazole, with a ratio of 63 % for C. albicans isolates and 16 % for Candida glabrata isolates. Moreover, relative quantification of genes related to resistance to fluconazole, CDR1, ERG11 as well as HWP1, showed a significant change compared to controls. Conclusion. Monitoring of vaginal Candida colonization before the third trimester of pregnancy, that could reduce congenital Candida colonization and risk of pregnancy complications.

Publisher

Microbiology Society

Subject

Microbiology (medical),General Medicine,Microbiology

Reference46 articles.

1. Candidal (vulvovaginitis);Lopez;BMJ Clin Evid,2015

2. Fungal diseases of vulva and vagina caused by Candida species;Stock;Med Monatsschr Pharm,2010

3. Die vaginale Kolonisation durch Candidaarten unter besonderer Berücksichtigung von Candida dubliniensis

4. Vulvovaginal Candidiasis in Pregnancy

5. Vulvovaginal candidosis

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