Litorimonas taeanensis gen. nov., sp. nov., isolated from a sandy beach

Abstract

A heterotrophic, Gram-negative, prosthecate bacterium, designated strain G5T, was isolated from a sandy beach of Taean in South Korea. Cells of strain G5T were aerobic, catalase- and oxidase-positive, straight to slightly curved motile rods with a single flagellum and formed yellow–orange colonies on agar. Growth occurred at 15–40 °C (optimum 25–30 °C) and pH 6–9 (optimum pH 7–8). The major cellular fatty acids were C18 : 1ω7c, C17 : 0, C16 : 0, 11-methyl C18 : 1ω7c, C17 : 1ω8c and C17 : 1ω6c. The polar lipid pattern indicated the presence of phosphatidylglycerol, monoglycosyldiglyceride, glucuronopyranosyldiglyceride and two unidentified glycolipids. The G+C content of the genomic DNA was 63.6 mol% and the major quinone was Q-10. Comparative 16S rRNA gene sequence analysis showed that strain G5T belonged to the branch containing the genera Hellea, Robiginitomaculum and Hypomonas within the family Hyphomonadaceae. Within this group, strain G5T was most closely related to Hellea balneolensis 26III/A02/215T with 95.8 % 16S rRNA gene sequence similarity. Based on its phylogenetic position and its phenotypic, chemotaxonomic and molecular properties, strain G5T represents a novel species of a novel genus of the family Hyphomonadaceae, for which the name Litorimonas taeanensis gen. nov., sp. nov. is proposed. The type strain is G5T ( = KACC 13701T  = DSM 22008T).