Streptomyces shaanxiensis sp. nov., a blue pigment-producing streptomycete from sewage irrigation soil

Abstract

A novel isolate belonging to the genus Streptomyces , strain CCNWHQ 0031T, was isolated from soil in a sewage irrigation area in Shaanxi province, China. The isolate produced light greyish-blue aerial mycelium and dark blue diffusible pigment on Gause’s synthetic agar. Strain CCNWHQ 0031T formed Spirales-type chains with spiny spores. Chemotaxonomic data confirmed that strain CCNWHQ 0031T belonged to the genus Streptomyces . Analysis of the almost complete 16S rRNA gene sequence placed strain CCNWHQ 0031T in the genus Streptomyces where it formed a distinct phyletic line with recognized Streptomyces species. Strain CCNWHQ 0031T exhibited highest sequence similarities to Streptomyces caeruleatus GIMN4.002T (99.3 %), Streptomyces coeruleorubidus NBRC 12855T (98.9 %), Streptomyces curacoi NBRC 12761T (98.8 %) and Streptomyces lincolnensis NBRC 13054T (98.0 %). DNA–DNA hybridization studies of strain CCNWHQ 0031T with these four closest relatives showed relatedness values of 56.6±0.4, 50.3±0.6, 49.8±0.7 and 36.9±0.9 %, respectively. On the basis of its molecular and physiological properties, it is evident that strain CCNWHQ 0031T represents a novel species of the genus Streptomyces , for which the name Streptomyces shaanxiensis sp. nov. is proposed. The type strain is CCNWHQ 0031T ( = CCNWTJ 0031T = JCM 16925T = ACCC 41873T).