Affiliation:
1. Guangxi Key Laboratory for Polysaccharide Materials and Modifications, School of Marine Sciences and Biotechnology, Guangxi Minzu University, Nanning 530008, China; No. 158, Daxue Xi Road, Nanning, PR China
2. Yunnan Institute of Microbiology, Yunnan University, Kunming 650091, China; No. 182, 121st Street, Kunming, PR China
Abstract
A Gram-stain-positive actinobacterium, designated strain GXMU-J5T, was isolated from a sample of shrimp pond soil collected in Tieshangang Saltern, Beihai, PR China. The morphological, chemotaxonomic and phylogenetic characteristics were consistent with its classification in the genus
Streptomyces
. The organism formed an extensively branched substrate mycelium, with abundant aerial hyphae that differentiated into spores. Phylogenetic analysis of 16S rRNA gene sequences showed that strain GXMU-J5T was most related to
Streptomyces kunmingensis
DSM 41681T (similarity 97.74 %) and
Streptomyces endophyticus
YIM 65594T (similarity 96.80 %). However, the values of digital DNA–DNA hybridization, average nucleotide identity and evolutionary distance of multilocus sequence analysis between strain GXMU-J5T and its closest relatives indicated that it represented a distinct species. Strain GXMU-J5T contained ll-diaminopimelic acid and the major whole-cell hydrolysates were xylose and galactose. The predominant menaquinones of strain GXMU-J5T were revealed as MK-9(H4), MK-9(H6) and MK-9(H8). The polar lipids consisted of diphosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol mannosides and phospholipids of unknown structure containing glucosamine. The predominant cellular fatty acids were iso-C15 : 0, anteiso-C15 : 0, iso-C16 : 0, iso-C17 : 0 and anteiso-C17 : 0. The whole genome size of strain GXMU-J5T was 6.79 Mbp with a 71.39 mol% G+C content. Genomic analysis indicated that strain GXMU-J5T had the potential to degrade chitin. On the basis of these genotypic and phenotypic data, it is supported that strain GXMU-J5T represents a novel species of the genus
Streptomyces
, for which the name Streptomyces beihaiensis sp. nov. is proposed. The type strain is strain GXMU-J5T (=MCCC 1K08064T=JCM 35629T).
Funder
Natural Science Foundation of Guangxi Province
InnovationTeam Fund Project of Young Xiangsi Lake Scholars of Guangxi Minzu University
Research on experimental technology innovation and Laboratory management of Guangxi Minzu Univesity
Guangxi Graduate Education Innovation Plan Project
Innovation Project of Guangxi Graduate Education
Natural Science Foundation of China
Science and Technology Major Project of Guangxi Province
Subject
General Medicine,Ecology, Evolution, Behavior and Systematics,Microbiology
Cited by
2 articles.
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