Affiliation:
1. School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, PR China
Abstract
Four extremely halophilic archaeal strains, LYG-108T, LYG-24, DT1T and YSSS71, were isolated from salted Laminaria produced in Lianyungang and saline soil from the coastal beach at Jiangsu, PR China. The four strains were found to be related to the current species of
Halomicroarcula
(showing 88.1–98.5% and 89.3–93.6% similarities, respectively) as revealed by phylogenetic analysis based on 16S rRNA and rpoB′ genes. These phylogenies were fully supported by the phylogenomic analysis, and the overall genome-related indexes (average nucleotide identity, DNA–DNA hybridization and average amino acid identity) among these four strains and the
Halomicroarcula
species were 77–84 %, 23–30 % and 71–83 %, respectively, clearly below the threshold values for species demarcation. Additionally, the phylogenomic and comparative genomic analyses revealed that
Halomicroarcula salina
YGH18T is related to the current species of
Haloarcula
rather than those of
Halomicroarcula
,
Haloarcula salaria
Namwong et al. 2011 is a later heterotypic synonym of
Haloarcula argentinensis
Ihara et al. 1997, and
Haloarcula quadrata
Oren et al. 1999 is a later heterotypic synonym of
Haloarcula marismortui
Oren et al. 1990. The major polar lipids of strains LYG-108T, LYG-24, DT1T and YSSS71 were phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester, phosphatidylglycerol sulphate, sulphated mannosyl glucosyl diether and additional glycosyl-cardiolipins. All these results showed that strains LYG-108T (=CGMCC 1.13607T=JCM 32950T) and LYG-24 (=CGMCC 1.13605=JCM 32949) represent a new species of the genus
Halomicroarcula
, for which the name Halomicroarcula laminariae sp. nov. is proposed; strains DT1T (=CGMCC 1.18928T=JCM 35414T) and YSSS71 (=CGMCC 1.18783=JCM 34915) also represent a new species of the genus
Halomicroarcula
, for which the name Halomicroarcula marina sp. nov. is proposed.
Funder
National Natural Science Foundation of China
Subject
General Medicine,Ecology, Evolution, Behavior and Systematics,Microbiology
Cited by
3 articles.
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