Affiliation:
1. Key Laboratory of Biology and Resources of Tropical Crops, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences & Key Laboratory of Biology and Resources of Tropical Crops of Hainan Province, Hainan institute for Tropical Agricultural Resources, Haikou, 571101, PR China
2. College of Public Health, South China University, Hengyang, Hunan 421001, PR China
Abstract
A novel actinobacterium strain (M4I6T) was isolated from marine sediment collected in Megas Gialos, Syros, Greece. On the basis of 16S rRNA gene sequence analysis, strain M4I6T was indicated as belonging to the genus
Actinoplanes
, with high similarity to ‘Actinoplanes solisilvae’ LAM7112T (97.9 %),
Actinoplanes ferrugineus
IFO 15555T (97.6 %),
Actinoplanes cibodasensis
LIPI11-2-Ac042T (97.2 %) and
Actinoplanes bogorensis
LIPI11-2-Ac043T (97.2 %). Phylogenetic analysis of the 16S rRNA gene sequence of strain M4I6T showed that the strain formed a stable subclade with ‘A. solisilvae’ LAM7112T. The cell wall of the novel isolate contained meso-diaminopimelic acid and the whole-cell sugars were xylose, glucose and ribose. The predominant menaquinones were MK-9(H4), MK-9(H2) and MK-9(H8). The phospholipid profile comprised phosphatidylethanolamine, phosphatidylinositol, diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol mannosides and an unknown phospholipid. The major fatty acids (>5 %) were anteiso-C16 : 0, iso-C17 : 0, 10-methyl-C16 : 0, C15 : 0, iso-C16 : 0 and C17 : 0. Genome sequencing showed a DNA G+C content of 70.9 mol%. However, the low average nucleotide identity value, digital DNA–DNA hybridization and average amino acid identity values demonstrated that strain M4I6T could be readily distinguished from its closest related species. Based on data from this polyphasic study, strain M4I6T represents a novel species of the genus
Actinoplanes
, for which the name Actinoplanes maris sp. nov. is proposed. The type strain is M4I6T (=DSM 101017T=CGMCC 4.7854T).
Funder
Natural Science Foundation of Hainan Province
Financial Fund of the Ministry of Agriculture and Rural Affairs, P. R. of China
China Agriculture Research System of MOF and MARA
Subject
General Medicine,Ecology, Evolution, Behavior and Systematics,Microbiology
Cited by
3 articles.
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