Affiliation:
1. State Key Laboratory of Development Biology of Freshwater Fish Sub-Center for Health Aquaculture, Hunan Provincial Key Laboratory for Molecular Immunity Technology of Aquatic Animal Diseases, Changde Key Innovation Team for Wetland Biology and Environmental Ecology, College of Life and Environmental Sciences, Hunan University of Arts and Science, Changde 415000, Hunan, PR China
2. School of Life and Health Sciences, Hunan University of Science and Technology, Xiangtan 411201, Hunan, PR China
Abstract
A novel Gram-stain-positive, endophytic actinobacterium, designated strain HDS5T, was isolated from leaves of Eucommia ulmoides Oliv. collected from Changde City, Hunan Province, PR China. Strain HDS5T produced yellowish oil green substrate mycelia on Gause’s synthetic medium, which also carried yellowish oil green aerial hyphae, fragmenting into rod-shaped elements with smooth surfaces. Strain HDS5T grew at pH 5.0–11.0 (optimum, pH 7), at 20–40 °C (optimum, 28 °C) and in the presence of 0–8.0% NaCl (w/v; optimum, 0–1.0 %). Whole-cell hydrolysates contained meso-diaminopimelic acid as the diagnostic amino acid and no diagnostic sugars. The predominant fatty acids were iso-C16:0 and C18 : 1
ω9c. The menaquinones were MK-10(H2), MK-10(H4) and MK-10(H6). Strain HDS5T showed high 16S rRNA gene sequence similarity to
Nocardiopsis prasina
DSM 43845T (99.72 %),
Nocardiopsis ganjiahuensis
DSM 45031T (99.31 %),
Nocardiopsis exhalans
JCM 11759T (99.17 %),
Nocardiopsis alba
DSM 43377T (99.11 %),
Nocardiopsis metallicus
KBS6T (99.11 %),
Nocardiopsis valliformis
DSM 45023T (99.04 %),
Nocardiopsis listeri
NBRC 13360T (98.97 %),
Nocardiopsis lucentensis
DSM 44048T (98.83 %),
Nocardiopsis terrae
YIM 90022T (98.83 %) and <98.7 % similarities to other type strains. Phylogenetic analysis of 16S rRNA gene sequences and whole-genome sequences showed that strain HDS5T was closely related to
N. prasina
DSM 43845T. However, the average nucleotide identity based on blast and digital DNA–DNA hybridization values between them were determined to be 90.1 and 40.9 %, respectively, below the threshold of 95–96 and 70 % for the delineation of prokaryotic genomic species, suggesting that strain HDS5T represents a novel
Nocardiopsis
species. Furthermore, the morphological and physio-biochemical characteristics were sufficient to distinguish strain HDS5T from
N. prasina
DSM 43845T. Consequently, based on phenotypic and genotypic characteristics, strain HDS5T represents a new
Nocardiopsis
species, for which the name Nocardiopsis eucommiae sp. nov. is proposed. The type strain is HDS5T (=MCCC 1K06172T=JCM 34707T).
Funder
Education Department of Hunan Province
Natural Science Foundation of Hunan Province
Key projects of Hunan Provincial Department of Education
Doctoral Start-up Foundation of Liaoning Province
Innovation Team of Microbial Technology in Hunan University of Arts and Science
Subject
General Medicine,Ecology, Evolution, Behavior and Systematics,Microbiology
Cited by
2 articles.
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