Taibaiella helva sp. nov., isolated from farmland soil in China

Abstract

A yellow-coloured bacterial strain, designated F-4T, was isolated from a farmland soil sample from Qianshan, Anhui province, China. Strain F-4T was Gram-stain-negative, strictly aerobic, oval-shaped, motile (by gliding) and non-spore-forming. Growth occurred at 20–35 °C (optimum, 30 °C), at pH 6.0–8.0 (pH 7.0) and with 0–1.0 % (w/v) NaCl (0 %). Phylogenetic analysis based on 16S rRNA gene sequences showed that strain F-4T belonged to the genus Taibaiella . 16S rRNA gene sequence similarity values between strain F-4T and the type strains of the three recognized species of the genus Taibaiella , Taibaiella koreensis KACC 17171T, Taibaiella soli KCTC 42277T and Taibaiella chishuiensis JCM 19637T, were 98.1, 96.4 and 95.9 %, respectively. The predominant respiratory quinone was MK-7, with MK-8 as a minor component. The major polar lipids of strain F-4T were three unidentified lipids, two unidentified aminolipids, three unidentified phospholipids, an unidentified aminophospholipid and phosphatidylethanolamine. The major cellular fatty acids were iso-C15 : 0, iso-C15 : 1 G and iso-C17 : 0. The G+C content of the genomic DNA based on total genome calculations was 51.3 mol%. The major polyamine of strain F-4T was homospermidine. The average nucleotide identity and the digital DNA–DNA hybridization values for draft genomes between strain F-4T and strain THG-DT86T were 79.8 and 22.6 %, respectively. On the basis of the genotypic and phenotypic data presented here, strain F-4T represents a novel species of the genus Taibaiella , for which the name Taibaiella helva sp. nov. is proposed. The type strain is F-4T (=KCTC 62442T=CGMCC 1.13562T).