Abstract
The natural compounds produced in plants are classified into two major groups (Primary and secondary metabolic compounds). These compounds are the precursor materials for thecompounds of the second group, which are represented by secondary metabolites, most of which produce from three main compounds: shikimic acid, acetate, and fatty acids. Primary metabolites are the basic units in the metabolism of secondary compounds. Tissue cultures of plants are used to produce large quantities of secondary metabolic products, although cultures of callus and cell suspensions often do not produce higher levels of the whole plant. Therefore, some technologies were used to increase the production of secondary metabolites by plant tissue culture techniques through the selection of high-production cells. The growth of plant cells in tissue cultures occurs when the requirements for division and growth are available for them from nutrients, growth regulators, and any other additives that all affect the metabolic activities within the cells. To achieve optimal productivity of secondary metabolites, it is preferable to produce cells in a medium that is optimal for increasing biomass. Plant growth regulators such as auxins and cytokinins affect cell division, various metabolic processes, and plant growth in tissue cultures.
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