Determination of Signal Molecules, Proteins, and Low-Molecular-Weight Organic Compounds in Wheat Varieties Infected by Leaf Rust Disease

Abstract

The disease resistance of wheat cultivars varies due to the plants’ defense systems, which include signaling molecules such as ferulic (coniferelic) acid, salicylic, arachidonic, and jasmonic acids. Therefore, the determination of quantitative and qualitative composition of signal molecules, low-molecular organic compounds, and proteins in wheat varieties with different leaf rust disease resistance levels is crucial. In this study, proteins and signal molecules in wheat leaf samples inoculated with fungal pathogen were isolated and identified using LCMS and HPLC chromatography. In addition, changes in the concentration of several organic compounds in infected plants were analyzed. The validity of obtained data on the absence of other components of metabolic cycles after chromatographic separation was discussed.