Abstract
Cattle is a monotocous species that generally produce only one offspring per conception. However, multiple ovulations are a naturally emerging reproductive phenomenon typically controlled by genetic structure and environmental factors. On the other hand, few genes or causative mutations might explain significant genetic variations between animals for the reproductive traits. Studies report different methods, including QTL analysis, fine mapping, GWAS, and MAS selection, to improve such traits due to their economic importance. The recent fine-mapping study, which narrows the genomic region, indeed, influencing multiple ovulation, gives positive signals that causative mutation controlling high ovulation rate may be identified shortly. In conclusion, identifying the major genes that considerably affect ovulation and twinning rates provides the opportunity to increase reproduction efficiency by improving genetic gain in livestock species.