Influence fatty acids on oxygen consumption by isolated rat cardiomyocytes in ischemic or diabetic heart disease

Abstract

Background: one of the reasons of violation of the functional viability of the myocardium is considered to be the shortage oxygen and energy of the myocardium. Its reason is the inhibitory effect of fatty acids on glucose oxidation. Recently, however, new data have been published, proving the need for and importance of fatty acids in the maintenance and regulation of the functional activity of the myocardium in chronic pathology.Objective: to investigate the influence of free polyunsaturated and saturated fatty acids (FA) on the oxygen uptake of isolated cardiomyocytes in intact rats and animals with ischemic or diabetic heart disease.Methods: executed non-randomized controlled study. Considered 3 groups of male rats of Wistar line (weight 250-300 g) with 10 animals in each group. Myocardial infarction ("heart attack") caused by ligation of the left coronary artery, and diabetes ("diabetes") – intraperitoneal injection of streptozotocin. The group "control" was presented intact animals. Myocardial infarction caused by ligation of the left coronary artery, and diabetes by intraperitoneal injection of streptozotocin. Isolated cardiac myocytes were obtained by enzymatic method. Oxygen consumption was assessed polarographically at different saturation incubation medium with oxygen ([O2] £8 mg/l and ([O2] ≥16 mg/l).Results: As the fatty acid used arachidonic and palmitic acid. It is established that the introduction to the incubation medium 20 μm arachidonic or palmitic fatty acid significantly increased the oxygen consumption of intact cardiomyocytes of rats. Both at the ischemic and at diabetic defeat of heart was obtained the opposite result. The most pronounced decrease of oxygen consumption are shown in diabetes mellitus.Conclusion: Conclude that the inhibitory effect of LCD on the rate of oxygen consumption during the at the ischemic and at diabetic defeat of heart may be due to their influence on the barrier function of mitochondrial membranes of cardiomyocytes, the activity of membrane-associated enzymes and their associated processes.