Author:
Azevedo Ricardo A.,Damerval Catherine,Lea Peter J.,Landry Jacques,Bellato Cláudia M.,Meinhardt Lyndel W.,Guilloux Martine Le,Delhaye Sonia,Toro Alejandro A.,Gaziola Salete A.,Varisi Vanderlei A.,Gratão Priscila Lupino
Abstract
The capacity of three maize endosperm opaque mutants (o10, o11 and o13) to accumulate soluble lysine in the seed in relation to their wildtype counterpart, W22+, was investigated. The W22o13 and W22o11 mutants exhibited 278% and 186% increases in soluble lysine, respectively, while for W22o10, a 36% decrease was observed, compared with the wildtype. A quantitative and qualitative study of the N constituents of the endosperm has been conducted and data obtained for the total protein, non-protein N, soluble amino acids, albumins / globulins, zeins and glutelins present in the seed of the mutants. Following 2D–PAGE, a total of 38 different forms of zein polypeptides were detected and considerable differences were noted between the three mutant lines. The metabolism of lysine was also studied by analysis of the enzymes aspartate kinase, homoserine dehydrogenase, lysine 2-oxoglutarate reductase and saccharopine dehydrogenase, which exhibited major changes in activity, depending on the genotype, suggesting that the mutant genes may have distinct regulatory activities.
Subject
Plant Science,Agronomy and Crop Science
Cited by
37 articles.
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