Author:
Comba María E.,Benavides María P.,Tomaro María L.
Abstract
The antioxidant defence systems of soybean (Glycine max
(L.) Merr) nodules responded differently to 50 and 200 mM NaCl. At 50 mM NaCl,
leghaemoglobin content and nitrogenase activity remained unaltered but there
was an overall increase in the antioxidant enzymes (ascorbate peroxidase,
catalase, glutathione reductase and superoxide dismutase) and in reduced
glutathione. After returning the salinised nodules to a non-saline environment
(recovery), the enzymatic activities returned to the initial values but
reduced glutathione remained high with respect to the controls measured at the
end of the experiment (final controls). Severe salt treatment reduced the
leghaemoglobin content and nitrogenase activity by 31% and 50%,
respectively. Ascorbate peroxidase, catalase and glutathione reductase
activities decreased between 30 and 100% while superoxide dismutase and
reduced glutathione increased over the controls by 19% and 30%
respectively. After recovery, glutathione reductase increased over the final
controls and reduced glutathione remained as under 50 mM NaCl. Malondialdehyde
content and total protein remained unchanged in nodules treated with the two
salt concentrations. These results suggest that under mild saline stress, the
elevated levels of the antioxidant enzymes and reduced glutathione protect
nodules against the activated oxygen species thus avoiding lipid and protein
peroxidation, and leghaemoglobin breakdown. However, severe saline treatment
produced an irreversible decay in the leghaemoglobin content and nitrogenase
activity despite the high reduced glutathione level and glutathione reductase
activity.
Subject
Plant Science,Agronomy and Crop Science
Cited by
78 articles.
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