Endosperm-specific OsPYL8 and OsPYL9 act as positive regulators of the ABA signaling pathway in rice seed germination

Abstract

Pyrabactin resistance-like (PYL) proteins were identified as receptors of the plant hormone ABA. The PYL family consists of multiple members that are differently expressed in various tissues, exhibit distinct biochemical properties and have diverse biological functions. In the present study, we explored the expression patterns of the rice (Oryza sativa L.) OsPYL family genes and determined that OsPYL8 and OsPYL9 are specifically expressed in the endosperms. Sequence analysis and deletion experiments revealed that the OsPYL8 and OsPYL9 promoters contain multiple motifs involved in endosperm-specific expression. Transgenic rice plants overexpressing OsPYL8 or OsPYL9 showed hypersensitivity to ABA during seed germination, suggesting that both OsPYL8 and OsPYL9 act as positive regulators of the ABA signalling pathway in the seed. OsPYL8 and OsPYL9 interact with OsPP2C51 and OsPP2C68, whose expression is induced in the endosperms by ABA. Our results provided a foundation for future studies on OsPYL8- and OsPYL9-mediated ABA signalling in the rice endosperms.