Cellular autofluorescence and browning in trichomes of Chinese cabbage (Brassica campestris) in response to mechanical stimulation and senescence

Abstract

There is limited information concerning the formation of dot-like browning appearing at the base of trichomes on mature leaves on the Chinese cabbage (Brassica campestris L. ssp. pekinensis). This study confirmed for the first time that enhanced autofluorescence can be induced in the base of trichomes when pressure stimuli is applied to trichomes; the enhanced autofluorescence gradually moves to the top of trichomes and the neighbouring mesophyll tissue within 15min. The excitation of autofluorescence in trichomes was found to be more effective in mature leaves compared to newly emergent leaves. Increased polyphenol oxidase (PPO) activities and reactive oxygen species (ROS) accumulation were also detected in the basal region of trichomes that were subjected to mechanical stimuli. Enhanced fluorescence was observed at the top of the trichomes in senescencing leaves. A browning in the base of the trichomes during leaf senescence was observed. In contrast, no browning occurred at the base of the trichomes in leaves that were subject to pressure stimuli. The blue fluorescence in the trichomes in senescent leaves arises mainly from the condensed cytoplasm. No direct evidence was able to prove that the enhanced autofluorescent substances in the trichomes during leaf senescence are the cause of the browning at the early growth stages.