Microsatellite profile, heterosygosity and fertility of sheep-Reference-Cited by-同舟云学术

Microsatellite profile, heterosygosity and fertility of sheep

Published:2024-05-01 Issue:04 Volume:24 Page:510-521
ISSN:1997-4868
Container-title:Agrarian Bulletin of the
language:en
Short-container-title:

Author:

Matyukov Valeriy¹,Zharikov Yakov¹,Kaneva L.

Affiliation:

1. Institut agrobiotehnologiy im. A. V. Zhuravskogo Federal'nogo issledovatel'skogo centra «Komi nauchnyy centr Ural'skogo otdeleniya Rossiyskoy akademii nauk»

Abstract

The purpose of the study was to analyze the microsatellite profile of sheep of different breeds and directions of productivity, to characterize them by reproductive abilities and to identify a possible relationship between the level of heterozygosity by microsatellite loci with some signs of fertility. Methodology. The object of the study were three groups of ewes: purebred Romanov breed (50R/50R, n = 31), two-breed crossbreeds of the Pechora semitonkorn with the Kuibyshev breed (50P/50K, n = 41) and three-breed crossbreeds obtained from crossing two-breed crossbreeds with the Romanov breed (25P25K/50R, n = 20). Each breed group of ewes, depending on the level of heterozygosity, was divided into three classes: modal – M0, with heterozygosity below the modal class – M– and above – M+. Results. The genetic distances between the 50P/50K and 50R/50R groups were established: FST = 0.390 ± 0.0062, DN = 0.242 ± 0.0479, 50P/50K and 25P/25K/50R: FST = 0.033 ± 0.0058, DN= 0.218 ± 0.0429, 50R/50R and 25P/25K/50R: FST = 0.022 ± 0.0058, DN = 0.127 ± 0.0292. According to all the analyzed reproductive characteristics, except for the survival rate of the offspring, Romanov sheep had a significant advantage over other groups. The 25P/25K/50R crossbreeds were characterized by an increased average observed heterozygosity by markers and a significantly higher average age of the beginning of reproduction, which was higher than the indicator characterizing the most late-maturing parent group. Inheritance of other reproductive traits in the 25P25K/50R group, excluding the age of onset and the rate of reproduction, was intermediate with some regression at 50P/50K. Scientific novelty. Scientific novelty. The possible influence of the level of average heterozygosity at 12 STR loci on the fertility of ewes was analyzed. Practical significance. In all groups of ewes, the rank of the reproductive characteristics of the modal class M0 was higher than that of M– and M+. The repeatability of the relationship of fertility indicators with heterozygosity by STR loci allows the use of microsatellite polymorphism to solve applied problems, in particular, to predict fertility within a population.

Publisher

Urals State Agrarian University

Reference23 articles.

1. Улимбашев М. Б., Кулинцев В. В., Селионова М. И. [и др.] Рациональное использование генофонда ценных пород животных с целью сохранения биологического разнообразия // Юг России: экология, развитие. 2018. Т. 13, № 2. С. 165–183. DOI: 10.18470/1992-1098-2018-2-165-183., Ulimbashev M. B., Kulincev V. V., Selionova M. I. [i dr.] Racional'noe ispol'zovanie genofonda cennyh porod zhivotnyh s cel'yu sohraneniya biologicheskogo raznoobraziya // Yug Rossii: ekologiya, razvitie. 2018. T. 13, № 2. S. 165–183. DOI: 10.18470/1992-1098-2018-2-165-183.

2. Colino-Rabanal V. J., Rodríguez-Díaz R., Blanco-Villegas M. J., Peris S. J., Lizana M. Human and Ecological Determinants of the Spatial Structure of Local Breed Diversity // Scientific Reports. 2018. Vol. 8. No. 1. Article number 6452. DOI: 10.1038/s41598-018-24641-3., Colino-Rabanal V. J., Rodríguez-Díaz R., Blanco-Villegas M. J., Peris S. J., Lizana M. Human and Ecological Determinants of the Spatial Structure of Local Breed Diversity // Scientific Reports. 2018. Vol. 8. No. 1. Article number 6452. DOI: 10.1038/s41598-018-24641-3.

3. Галинская Т. В., Щепетов Д. М., Лысенков С. Н. Предубеждение о микросателлитных исследованиях и как им противостоять // Генетика. 2019. T. 55, № 6. С. 617–632. DOI: 10.1134/S0016675819060043., Galinskaya T. V., Schepetov D. M., Lysenkov S. N. Predubezhdenie o mikrosatellitnyh issledovaniyah i kak im protivostoyat' // Genetika. 2019. T. 55, № 6. S. 617–632. DOI: 10.1134/S0016675819060043.

4. Van der Westhuizen L., MacNeil M. D., Scholtz M. M., Neser F. W., Makgahlela M. L., van Wyk J. B. Genetic Variability and Relationships in Nine South African Cattle Breeds Using Microsatellite Markers // Tropical Animal Health and Production. 2020. Vol. 52, No. 1. Pp. 177–184. DOI: 10.1007/s11250-019-02003-z., Van der Westhuizen L., MacNeil M. D., Scholtz M. M., Neser F. W., Makgahlela M. L., van Wyk J. B. Genetic Variability and Relationships in Nine South African Cattle Breeds Using Microsatellite Markers // Tropical Animal Health and Production. 2020. Vol. 52, No. 1. Pp. 177–184. DOI: 10.1007/s11250-019-02003-z.

5. Svishcheva G., Babayan O., Lkhasaranov B., Tsendsuren A., Abdurasulov A., Stolpovsk Y. Microsatellite Diversity and Phylogenetic Relationships among East Eurasian Bos taurus Breeds with an Emphasis on Rare and Ancient Local Cattle // Animals. 2020. Vol. 10, No. 9. Article number 1493. DOI: 10.3390/ani10091493., Svishcheva G., Babayan O., Lkhasaranov B., Tsendsuren A., Abdurasulov A., Stolpovsk Y. Microsatellite Diversity and Phylogenetic Relationships among East Eurasian Bos taurus Breeds with an Emphasis on Rare and Ancient Local Cattle // Animals. 2020. Vol. 10, No. 9. Article number 1493. DOI: 10.3390/ani10091493.