Inducing Chondrogenic Differentiation in ATDC5 Cells Using a Three-Dimensional Hydrogel with GAG-Mimicking Properties

Abstract

This study aims to develop a method using a three-dimensional hydrogel that mimics glycosaminoglycans to accelerate the development of cartilage cells. The hydrogel contains a specific glycosaminoglycan-like peptide sequence with the potential to enhance the effectiveness of chondrogenic differentiation and provide a more efficient approach for tissue engineering applications. In the study, ATDC5 cells were cultured within a synthetic scaffold containing PA nanofibers that mimic the structure of glycosaminoglycans in a three-dimensional form. Cellular characterizations were conducted to induce chondrogenic differentiation. ATDC5 cells cultured on GAG-mimicking peptide nanofibers expressed cartilage-specific extracellular matrix components statistically significantly over a 14-day period compared to cells cultured on TCP without insulin induction. Amphiphilic peptide nanofibers offer a valuable approach to replicate glycosaminoglycan properties and support chondrogenic differentiation in ATDC5 cells without the need for growth factors or external stimuli. This approach holds substantial potential for clinical applications in cartilage tissue engineering.