Imaging Transcription in Living Cells

Author:

Darzacq Xavier12,Yao Jie13,Larson Daniel R.14,Causse Sébastien Z.12,Bosanac Lana12,de Turris Valeria14,Ruda Vera M.12,Lionnet Timothee14,Zenklusen Daniel14,Guglielmi Benjamin13,Tjian Robert13,Singer Robert H.14

Affiliation:

1. Janelia Farm Research Consortium on Imaging Transcription, Janelia Farm Research Campus, Howard Hughes Medical Institute, Ashburn, Virginia 20147

2. Imagerie Fonctionelle de la Transcription, Ecole Normale Supérieure CNRS UMR 8541, 75230 Paris cedex 05, France;

3. Howard Hughes Medical Institute, Department of Molecular and Cell Biology, University of California, Berkeley, California 94720;

4. Department of Anatomy and Structural Biology, Gruss-Lipper Biophotonics Center, Albert Einstein College of Medicine of Yeshiva University, Bronx, New York 10461;

Abstract

The advent of new technologies for the imaging of living cells has made it possible to determine the properties of transcription, the kinetics of polymerase movement, the association of transcription factors, and the progression of the polymerase on the gene. We report here the current state of the field and the progress necessary to achieve a more complete understanding of the various steps in transcription. Our Consortium is dedicated to developing and implementing the technology to further this understanding.

Publisher

Annual Reviews

Subject

Cell Biology,Biochemistry,Bioengineering,Structural Biology,Biophysics

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