Molecular characterization of Trichogramma spp. in sugarcane (Saccharum officinarum) ecosystem in Punjab-Reference-Cited by-同舟云学术

Molecular characterization of Trichogramma spp. in sugarcane (Saccharum officinarum) ecosystem in Punjab

Published:2023-07-07 Issue:6 Volume:93 Page:
ISSN:2394-3319
Container-title:The Indian Journal of Agricultural Sciences
language:
Short-container-title:Indian J Agri Sci

Author:

KAUR JASREET,JINDAL VIKAS,SANGHA KAMALDEEP SINGH

Abstract

Trichogramma chilonis (Ishii) is of great importance to biological control of many harmful lepidopteran pests of crops. There is a need to establish the species composition in the fields for differentiation among the species/strains in order to enhance efficiency of their practical application. This study aimed to catalogue the species of Trichogramma available in sugarcane in Punjab. The populations were collected from different districts of Punjab, viz. Gurdaspur, Shaheed Bhagat Singh Nagar, Patiala, Amritsar and Fazilka during 2017–18. The mtCOI gene of Trichogramma was amplified and sequenced for identification of species and assessing the genetic diversity. Nucleotide sequences of Trichogramma populations collected from different sugarcane growing districts of Punjab showed 99% homology with T. chilonis. Multiple alignments of mtCOI sequences showed three branches with Punjab populations similar to New Delhi, Bengaluru and Pakistan. This study confirms the successful establishment of T. chilonis in sugarcane ecosystem in Punjab.

Publisher

Indian Council of Agricultural Research, Directorate of Knowledge Management in Agriculture

Subject

Agronomy and Crop Science

Reference13 articles.

1. Chakraborty K. 2011. Report on egg parasitoids of Scirpophaga incertulas from Hemtabad, West Bengal. Annals of Plant Protection Sciences 19: 213–14.

2. Folmer O, Black M, Hoeh W, Lutz R and Vrijenhoek R. 1994. DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates. Molecular Marine Biology and Biotechnology 3: 294–99.

3. Kaur R and Brar K S. 2008. Evaluation of different doses of Trichogramma species for the management of leaf folder and stem borer on basmati rice. Journal of Biological Control 22: 131–35.

4. Monaghan M T, Balke M, Gregory T R and Vogler A P. 2005. DNA-based species delineation in tropical beetles using mitochondrial and nuclear markers. Philosophical Transactions of the Royal Society of London Biological Sciences 360: 1925–33.

5. Nagaraja H and Mohanraj P. 2010. Three new species of Trichogramma (Hymenoptera: Trichogrammatidae) from southern India. Journal of Biological Control 24: 203–09.