Author:
GHODE GAJANAN,RATHORE GAURAV,TRIPATHI GAYATRI,DAVANE KASHMIRA,PRASAD KURUCHETI PANI
Abstract
Toll like receptor (TLR) signalling pathway plays a central role in inflammatory responses through the recognition of PAMPs and DAMPs. Toll-like receptor 4 (TLR4) is well known for the recognition of LPS of gram-negative bacteria leading to production of inflammatory cytokines. Modulation of a TLR pathway gene in different tissues indicates the functional importance of the tissues in innate immunity. The aim of this study was to clone and characterize TLR4 and evaluate its constitutive tissue distribution in Pangasianodon hypophthalmus, a commercially important fish species. The study was carried out at ICAR-CIFE, Mumbai during 2017. Partial nucleotide and amino acid sequences of TLR4 were identified in P. hypophthalmus and termed as phTLR4. Bio-informatics analysis of partial sequence revealed four putative LRR domains characteristic of TLRs. Nucleotide and amino acid sequences showed varying degree of identity with TLR4 sequences from other fish species in BLASTn and BLASTp analysis. Phylogenetic analysis placed the phTLR4 close to Ictalurus punctatus. TLR4 was found to be constitutively expressed in all the tissues studied. The significantly highest expression was recorded in intestine and spleen. The lowest expression was in muscle, heart and skin. House-keeping gene ß-actin was used as an internal control. The outcome of this research will be helpful for studying mRNA expression levels of phTLR4 under different conditions in different tissues of Pangasianodon hypophthalmus.
Publisher
Indian Council of Agricultural Research, Directorate of Knowledge Management in Agriculture
Subject
General Veterinary,Animal Science and Zoology
Reference35 articles.
1. Arancibia S A, Beltrán C J, Aguirre I M, Silva P, Peralta A L, Malinarich F and Hermoso M A. 2007. Toll-like receptors are key participants in innate immune responses. Biological Research 40(2): 97–112.
2. Basu M, Kumar M N and Samanta M. 2013. Toll-like receptor (TLR) 4 in mrigal (Cirrhinus mrigala): response to lipopolysaccharide treatment and Aeromonas hydrophila infection. International Research Journal of Biological Sciences 2(4): 20–27.
3. Basu M, Paichha M, Swain B, Lenka S S, Singh S, Chakrabarti R and Samanta M. 2016. Modulation of TLR2, TLR4, TLR5, NOD1 and NOD2 receptor gene expressions and their downstream signaling molecules following thermal stress in the Indian major carp catla (Catla catla). Biotech 5: 1021–30.
4. Chen K, Zhao F, Ouyang G, Shi Z, Ma L, Wang B, Guo R, Xiao W, Zhu F, Wei K and Xu Z. 2021. Molecular characterization and expression analysis of Tf_TLR4 and Tf_TRIL in yellow catfish Tachysurus fulvidraco responding to Edwardsiella ictaluri challenge. International Journal of Biological Macromolecules 167: 746–55.
5. Dhanasekaran S, Kannan T A, Dhinakar Raj G, Tirumurugaan K G, Raja A and Kumanan K. 2010. Differential expression of Toll‐like receptor mRNA in corneal epithelium of ruminants. Veterinary Ophthalmology 13(4): 270–74.