Effects of a Heavy-Atom Salt on the Solid-Matrix Luminescence Properties of the (±)-Trans-7,8-Dihydroxy-Anti-9,10-Epoxy-7,8,9,10-Tetrahydrobenzo[a]Pyrene-DNA Adducts and Tetrol I-1 Adsorbed on Several Solid Matrices

Abstract

The effects of a heavy-atom salt on the room-temperature solid-matrix luminescence properties of the (±)- trans-7,8-dihydroxy- anti-9,10-epoxy-7,8,9,10-tetrahydrobenzo[ a]pyrene-DNA adducts [(±)- anti-BPDE-DNA adducts] and benzo[ a]pyrene- r-7, t-8,9, c-10-tetrahydrotetrol (tetrol I-1) on several solid matrices were investigated. TlNO3 was found to be very effective in enhancing the room-temperature phosphorescence of the (±)- anti-BPDE-DNA adducts and tetrol I-1. However, the room-temperature fluorescence signals were quenched from the (±)- anti-BPDE-DNA adducts and tetrol I-1 on the solid matrices with the heavy-atom salt. It was found that 1% α-cyclodextrin/TlNO3, 10% trehalose/TlNO3, and 30% TlNO3/sodium acetate (NaOAc) yielded very strong RTP signals from the (±)- anti-BPDE-DNA adducts. The greatest enhancement of the RTP intensities from the (±)- anti-BPDE-DNA adducts was achieved with 30% TlNO3/NaOAc. In addition, a very low limit of detection (0.14 fmole/mg) for tetrol I-1 was acquired with 30% TlNO3/NaOAc. With its tremendous enhancement of RTP signals from the adducts and tetrol I-1 and the sub-femtomole limit of detection obtained for tetrol I-1, 30% TlNO3/NaOAc is ideal for the characterization and analysis of DNA adducts and their tetrol products.