Preparation and Evaluation of Florfenicol Nanocrystals for in Vivo Intestinal Absorption

Abstract

Initiatives to avoid or reduce the use of antibiotics have a positive significance, and maximizing the retention of the effectiveness of existing antibiotics is one of the effective measures that can be taken in the field of food animal husbandry. A "top-down" method was used to prepare florfenicol nanocrystals, which appeared as irregular prismatic structures under electron microscopy. The particle size measured by laser diffraction was (291.9±2.9) nm, and the zeta potential value was - (23.63±1.64) mV. The absorption results in vivo showed that florfenicol was absorbed in the duodenum, jejunum, ileum, and colon, but primarily in the duodenum. The effective permeability coefficient (Peff) of florfenicol increased to 1.8 times that of the bulk drug group after being prepared as nanocrystals. The above research results indicate that nanocrystal technology has a promoting effect on the intestinal absorption of florfenicol.