Phytochemical and pharmacological study of biologically active compounds and dry extracts of Populus rubrinervis Hort. Alb. buds of various polarities

Abstract

The aim of the work was a phytochemical and pharmacological study of biologically active compounds (BACs) and Populus rubrinervis Hort. Alb. buds preparations of various polarities.Materials and methods. The object of the study was dry extracts of P. rubrinervis Hort. Alb. buds the samples of which were prepared in January–March 2023 in the Botanical Garden of Samara University (Samara, Russia). The separation of the amount of current substances was carried consecutively by the method of circulating extraction (chloroform), then, by the method of fractional percolation, a tincture was received on 70% ethyl alcohol (1:5). Pinostrobin was used as the standard sample (SS). The analysis of the substances was carried out by the TLC method. The electronic spectra registration was carried out with a spectrophotometer “Specord 40” (Analytik Jena, Germany). The study of the pharmacological (diuretic) activity of P. rubrinervis Hort. Alb. buds dried extracts was carried out on 60 white outbred rats of both sexes weighing 200–220 g in the experiments with aqueous diuresis.Results. P. rubrinervis Hort. Alb. buds dried extracts of various polarities (extract No. 1 (chloroform) and extract No. 2 (70% ethanol) were received. By the method of thin-layer chromatography, it was determined that the dominant complexes of the lipophilic nature with pinostrobin are isolated in extract No. 1, phenolic substances of the glycoside nature prevail in extract No. 2. Despite various polarities of the extragents, spectral characteristics of extract No. 2 have significant similarities with extract No. 1. When studying the diuretic activity, it was established that when SS pinostrobin was injected at a dose of 1 mg/kg, for 4 h of the experiment, an isolated increase in diuresis was noted (from 1.72±0.11 to 1.97±0.03 ml, p <0.05); at the same time, an isolated increase in creatininuresis (from 1.50±0.29 to 2.39±0.15 mg, p <0.05) was observed during 24 h of the experiment. When extract No. 2 was injected at a dose of 10 mg/kg, there was a moderate significant increase in diuresis (from 1.82±0.02 to 2.07±0.04 ml and from 2.38±0.39 to 3.02±0.11 ml, p <0.05) and a significant increase in creatininuresis (from 0.14±0.01 to 0.19±0.03 mg and from 2.31±0.42 to 2.79±0.51 mg, p <0.05) for 4 and 24 h of the experiment, respectively.Conclusion. The extraction separation of the amount of P. rubrinervis Hort. Alb. buds by the polarity degree was carried out. Pinostrobin SS at a dose of 1 mg/kg and extract No. 2 at a dose of 10 mg/kg had a diuretic activity, in connection with which they are promising in terms of the development of effective drugs.