MASS SPECTROMETRY OF GLYCYRRETHENIC ACID COMPLEXES WITH STRIPTOMYCINES

Abstract

For the first time, complexes of glycyrrhetinic acid (GLA) with the antibiotic streptomycin (ST) have been studied. The complexes, obtained at different molar ratios of "host - GLA: guest - ST": 1 : 1, 2 : 1, 3 : 1 and 4 : 1, were studied using both spectrophotometry and electrospray ionization mass spectrometry. With increasing GLA concentration using spectrophotometry, the bathochromic shift of the maximum absorption of the MFR (203–210 nm) and the formation of an additional peak (260–273 nm) in the ST absorption spectrum are observed, indicating the formation of supramolecular complexes of different glycyrrhetinic acid stoichiometry with streptomycin antibiotic. Using electrospray ionization mass spectrometry, signals of nGLK-ST heterocomplexes containing GLA and CTP in the ratio of 1 : 1, 2 : 1, 3 : 1 and 4 : 1 were recorded, respectively. It has been shown that with increasing GLA concentration, the supramolecular structures are enlarged due to an increase in the number of GLA molecules in its composition. The presented data can be used in the development of a new dosage form of streptomycin based on supramolecular complexes with GLA.