COMPONENT COMPOSITION OF PHENOLIC COMPOUNDS AND TERPENOIDS OF THE ANGIOPROTECTIVE HERBAL COMPOSITION

Abstract

The study of the component composition of phenolic and terpenoid compounds of the angioprotective herbal composition was carried out. Alcohol extraction of the angioprotective herbal composition was studied by MC-HPLC-UV method. The analysis used solutions of commercial samples of reference substances manufactured by Sigma-Aldrich (USA), Chem-Fages, Extrasynthese, Lione (France), Beijing (China). Seven phenolic compounds have been identified: quercetin, isoramnetin, rutin, isoquercitrin, narcissin, isoramnetin-3-O-glucoside, phenolcarboxylic acid 5-O-caffeylquinic. The total content of flavonoids is 11.64 mg/g, phenolcarboxylic acid – 2.30 mg/g. Among the isolated flavonoids rutin (3.35±0.06 mg/g), isoquercitrin (3.14±0.06 mg/g), narcissin (4.15±0.09 mg/g), phenolcarboxylic acid – 5-O-caffeylquinic (2.30±0.05 mg/g) prevail. The essential oil was obtained by hydrodistillation; analysis was performed by gas chromatography-mass spectrometry on an Agilent Technologies (6890N) instrument with a quadrupole mass spectrometer. The identified components were performed by comparing the linear retention indices and total mass spectra of the compounds with the data from the Nist 11 library and commercial samples. In the herbal composition of the essential oil, 21 components have been identified, the main of which are salicylic aldehyde (in the total amount is 58.30%), methyl salicylate (16.17%). The triterpene saponins of the angioprotective herbal composition are represented by calendulosides A and B, escin. The amount of triterpene saponins is 1.08±0.05%. The results of the quantitative analysis were processed statistically, the data are presented as the mean and ± standard deviation, SD.