NGS-sequencing (Illumina) as a tool for determining the genomic composition and taxonomic affiliation of species and interspecific hybrids on the example of Hordeeae grasses

Abstract

The results of the study of intragenomic polymorphism of multicopy nuclear ITS1 DNA sequences in different representatives of the cereal genera of the tribe Hordeeae (Agropyron, Hordeum, Elytrigia, Elymus, Psathyrostachys, Leymus) and intergeneric hybrid x Leymotrigia by NGS (Illumina) are discussed. It is shown that this method can be a good tool for determining the genomic composition and origin of polyploid species and distant hybrids, as well as solving taxonomic questions about the genus and species status of objects.