Histochemical approaches to the evaluation of the participation of mast cells in the regulation of the fibrous component of the intercellular matrix of skin connective tissue

Abstract

The article presents the data of study of the effectiveness of various combinations of histochemical staining to determine the co-location of mast cells with a fibrous component of the connective tissue of Wistar line laboratory rats skin. The best results are obtained with combination protocols such as metachromatically detection of mast cells in combination with the impregnation with silver or picrofuchsin, brilliant green coloring with the methodology of Van Gieson or picrofuchsin, and aniline blue neutral red. The proposed variants of histochemical protocols can be used to analyze the participation of mast cells in the remodeling of the cell matrix of tissue microenvironment in the development of adaptive and pathological processes. The obtained results contribute to the expansion of currently available scientific ideas about the laws of the process of fibrillogenesis in the extracellular matrix and provide new data on the targeted participation of the components of the mast cell secretome in the molecular mechanisms of fiber formation.