Evaluation of the application and effects of intratesticular use of vaseline and acetylsalicylic acid 30% + vaseline for chemical castration in male rats

Abstract

The aim of this study was to evaluate the application and effects of intratesticular use of a mixture containing vaseline and 30% acetylsalicylic acid for chemical castration in male rats. Twenty–eight male albino Wistar rats were divided into four groups: control, sham, vaseline, and vaseline + 30% salicylic acid mixture. The control group (K) received no injection, the Sham group (S) was injected with 1 mL of saline, the vaseline group (V) was injected with 1 mL of vaseline, and the vaseline + acetylsalicylic acid group (V+ASA) was injected with 1 mL of a mixture containing 30% acetylsalicylic acid and vaseline. A statistically significant difference (P=0.000) was determined between the vaseline (V) and vaseline + acetylsalicylic acid (V+ASA) groups. The Malondialdehyde (MDA) value of the vaseline group was found to be higher (3.197 ± 0.08) with a statistically significant difference (P=0.000) compared to the other groups. In terms of glutathione (GSH) value, a statistically significant difference (P<0.05) was observed in the V+ASA group compared to the V and K groups. When comparing glutathione peroxidase (GSH–Px) levels. There was a significant difference (P=0.013) between the vaseline (V) and control (K) groups. A statistically significant difference (P=0.046) was found between the vaseline (V) and vaseline + acetylsalicylic acid (V+ASA) groups in terms of catalase (CAT) activities. In terms of motility in spermatological examination, it was determined that the vaseline (V) group had the lowest rate with 11,250 ± 3.14, showing a statistically significant difference (P=0.001) compared to the other three groups. The group with the highest sperm concentration was K (82,000 ± 6.60), while the lowest concentration was observed in group V (27,600 ± 3.54). The dead/viable ratio in semen was found to be the lowest (7,200 ± 2.03) in group K and the highest (61,250 ± 13.16) in group V. The highest rate and statistical difference in a chromosome values were determined in the vaseline (V) group. Histopathologically, the morphology of the testicular tubules was impaired in the vaseline (V) and vaseline + acetylsalicylic acid (V+ASA) groups, observed as degenerated and necrotic. Degenerated tubules were observed, devoid of germinative epithelial cells and consisting only of basal membrane. In conclusion, the castration process was performed irreversibly in the vaseline (V) and vaseline + acetylsalicylic acid (V+ASA) groups.