MOLECULAR-GENETIC CHARACTERIZATION OF FIELD ISOLATES OF RABIES VIRUS IDENTIFIED IN THE TERRITORY OF VLADIMIR, MOSCOW, TVER, NIZHNY NOVGOROD AND RYAZAN REGIONS

Author:

Zaykova O. N.1,Grebennikova T. V.1,Gulyukin A. M.2,Shabeykin A. A.2,Polyakova I. V.2,Metlin A. E.3

Affiliation:

1. D.I. Ivanovsky Institute of Virology, Federal State Budgetary Institution «Federal Research Centre for Epidemiology and Microbiology named after the honorary academician N.F. Gamaleya»; Institute for Medicine, Peoples' Friendship University of Russia

2. Y.R. Kovalenko All-Russian Scientific Research Institute of Experimental Veterinary

3. Federal Center for Animal Health

Abstract

The article presents a molecular genetic study of genomes of field isolates of rabies virus isolated in the Vladimir, Moscow, Tver, Nizhny Novgorod and Ryazan regions, with the aim of carrying out phylogenetic analysis. We studied 20 samples of purified PCR products containing the rabies virus nucleoprotein. The samples were provided by the Vladimir veterinary service. Sequencing and phylogenetic analysis of the gene showed that 12 fragments of isolates under study were close to the Central phylogenetic group of the rabies virus; namely - 5 isolates from the Vladimir region, 2 from the Nizhny Novgorod region, 2 from the Moscow region, and 3 from the Tver region. Eight studied isolates from the Nizhny Novgorod and Ryazan regions were attributed to the Eurasian phylogenetic group.

Publisher

Central Research Institute for Epidemiology

Subject

Infectious Diseases,Virology,General Medicine

Reference15 articles.

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4. Shabeikin A.A., Zaikova O.N., Gulyukin A.M. Obzor epizooticheskoi situatsii po beshenstvu v rossiiskoi federatsii za period s 1991 po 2015 gody. Veterinariya Kubani. 2016; (4): 4-6.

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