Affiliation:
1. FSBIS Kirov scientific-research institute of hematology and blood transfusion of FMBA of Russia
Abstract
Using data obtained from domestic and foreign sources, we formed a set of primers and fluorogenic probes for analyzing twenty-six specific sequence polymorphisms and one reference gene. In the course of evaluating the effectiveness of real-time PCR, using the example of one of the markers (S01a), we obtained the optimal amount of DNA per reaction (70 ng), providing a resolution of at least 0.1% of the method with the ability to estimate linear chimerism. Formed panel of primers for genetic polymorphisms - InDel has a high degree of informational content for donor-recipient pairs of Russia. From January 2018 to June 2019, a quantitative assessment of the level of linear (CD3 +, CD34 +) and general chimerism was carried out for 28 patients of the clinic of the Institution. Finally, we analyzed patients who received allografts and present 4 different clinical situations that illustrate the informativity level of this method.
Subject
Biochemistry, medical,Medical Laboratory Technology,General Medicine
Reference17 articles.
1. Lukanov T., Ivanova-Shivarova M., Naumova E. Chapter 4. Monitoring of Chimerism Following Hematopoietic Stem Cell Transplantation. In: Stem Cells in Clinical Practice and Tissue Engineering, Rijeka: IntechOpen; 2018: 77-93.
2. Alizadeh M., Bernard M., Danic B., Dauriac C., Birebent B., Lapart C. et al. Quantitative assessment of hematopoietic chimerism after bone marrow transplantation by real-time quantitative polymerase chain reaction. Blood. 2002; 99(12): 4618-25.
3. Koldehoff M., Steckel N.K., Hlinka M., Beelen D.W., Elmaagacli A.H. Quantitative analysis of chimerism after allogeneic stem cell transplantation by real-time polymerase chain reaction with single nucleotide polymorphisms, standard tandem repeats, and Y-chromosome-specific sequences. Am. J. Hematol. 2006; 81(10): 735–46.
4. Scheffer P.G., van der Schoot C.E., Page-Christiaens G.C., Bossers B., van Erp F., de Haas M. Reliability of fetal sex determination using maternal plasma. Obstet Gynecol. 2010; 115(1): 117-26.
5. Lavrinenko V.A., Savitskaya T.V., Volochnik E.V., Mareyko Yu.E., Aleynikova O.V. Quantitative analysis of chimerism after allogeneic hematopoietic stem cell transplantation with molecular genetic methods. Onkogematologiya. 2014; 9 (2): 29-36. (in Russian)
Cited by
1 articles.
订阅此论文施引文献
订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献