Studies on Effect of Antioxidant Enzymes Salicylic Acid and Jasmonic Acid Treated Plants of Acalypha indica.L

Abstract

Acalypha indica.L one of the traditional medicinal small annual shrubs belongs to the Euphorbiaceous Family, it grows up to 30-75 cm in height and is distributed in wet, temperate and tropical areas. It is available in gardens, road sides and throughout India. In the present work, we try to assess the antioxidant enzymes activity of SA & JA treated and control plants of Acalypha. Plants were treated with alone and different concentration combinations of SA & JA. After 55 days treated plants leaves were used for extractions. For extracting antioxidant enzymes, fresh leaves (0.5 gr) were ground using a tissue grinder in 5 m L of 50 mM cooled phosphate buffer (ph 7.8) placed in an ice bath. The homogenate was centrifuged at 15000 rpm for 20 min at 4 0C. The supernatant was used for determining the activities of enzymes. In this we find the antioxidant enzymes are Proline, superoxide dismutase (SOD), Glutathione reductase (GR), Ascorbic peroxidase (APX), Glycine betaine (GB). Hightest antioxidant enzyme activity was observed at the combination of T8 (3mM SA + 2μM JA) and lowest in T6 (1 mM SA+2μM JA). All alone and combination of SA & JA treated plants were contains high antioxidant enzyme activity, when compared to control one.