Effect of auxin plant hormones on growth and biochemical composition of microalgae Phaeodactylum tricornutum Bohlin, 1897-Reference-Cited by-同舟云学术

Effect of auxin plant hormones on growth and biochemical composition of microalgae Phaeodactylum tricornutum Bohlin, 1897

Published:2023-10-13 Issue:3 Volume:2023 Page:97-105
ISSN:2073-5529
Container-title:Vestnik of Astrakhan State Technical University. Series: Fishing industry
language:en
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Author:

Kovalev Nikolai Nikolayevich¹,Leskova Svetlana Evgenyevna¹,Mikheev Evgeny Valerevich¹

Affiliation:

1. Far Eastern State Technical Fisheries University

Abstract

Regulation of the growth rate of Phaeodactylum tricornutum opens up prospects for the organization of efficient industrial production of live feed biomass. We studied the effect of two plant hormones, indole-3-butyric acid (3-IBA) and indole-3-acetic acid (3-IAA), on the growth and biochemical composition of Ph. tricornutum in an enrichment culture. The effective 3-IBA concentration of 0.2 ∙ 10–5 M increased the density of the culture by 373% on day 14of cultivation compared to the control. High concentrations of 3-IAA either inhibited the development of the culture or did not have a significant effect on its growth. A stimulating effect of exposure to 3-IAA at 0.1 ∙ 10–5 M on the growth was recorded on day 12 of cultivation and amounted to 328.2% compared to the control. A stimulating effect of 3-IBA exposure on protein accumulation was recorded on day 4 of cultivation and amounted to 27.9% compared to the control. A stimulating effect of 3-IAA on protein synthesis was recorded on day 12 of cultivation of Ph. tricornutum. The protein concentration during this period was 1.2-fold higher than in the control. Indole-3-butyric acid did not have any effect on lipid accumulation, compared to the control, throughout the cultivation time. On day 14 of cultivation under the exposure to 3-IAA, the concentration of lipids was 1.5-fold higher than in the control and in the group exposed to 3-IBA. Indole-3-butyric acid did not show any effect on the accumulation of carbohydrates in the Ph. tricornutum culture throughout the cultivation period. On day 4 of cultivation, the carbohydrate concentration in the culture exposed to 3-IAA increased by 240% compared to the control. At the end of the experiment (14 days), no significant differences between the experi-mental cultures in the carbohydrate concentration were observed. For 8 days of Ph. tricornutum cultivation, the chlorophyll concentration increased by 927.4 and 1 178.6% as a result of exposure to 3-IBA and 3-IAA, respec-tively.

Publisher

Astrakhan State Technical University

Subject

General Medicine

Reference26 articles.

1. The State of World Fisheries and Aquaculture. Meeting the sustainable development goals. Rome: FAO, 2018. 227 p. URL: https://www.fao.org/3/I9540EN/i9540en.pdf (дата обращения: 13.04.2023)., The State of World Fisheries and Aquaculture. Meeting the sustainable development goals. Rome: FAO, 2018. 227 p. URL: https://www.fao.org/3/I9540EN/i9540en.pdf (data obrascheniya: 13.04.2023).

2. Helm M. M., Bourne N., Lovatelli A. Hatchery cul-ture of bivalves: a practical manual. FAO Fisheries Tech-nical Paper 471. Food and Agriculture Organization of the in the laboratory // Marine Biology. 2004. V. 15. P. 350–355., Helm M. M., Bourne N., Lovatelli A. Hatchery cul-ture of bivalves: a practical manual. FAO Fisheries Tech-nical Paper 471. Food and Agriculture Organization of the in the laboratory // Marine Biology. 2004. V. 15. P. 350–355.

3. Utting S. D., Spencer B. E. The Hatchery Culture of Bivalve Mollusc larvae and juveniles // Ministry of Agriculture, Fisheries and Food, Directorate of Fisheries Research Laboratory leaflet number. 1991. V. 68. P. 32., Utting S. D., Spencer B. E. The Hatchery Culture of Bivalve Mollusc larvae and juveniles // Ministry of Agriculture, Fisheries and Food, Directorate of Fisheries Research Laboratory leaflet number. 1991. V. 68. P. 32.

4. Kovalev N. N., Leskova S. E., Mikheev E. V. Hetoceros: modulation of growth and composition under the action of salicylic acid // Agrarnaya Rossia. 2022. N. 10. P. 13–18., Kovalev N. N., Leskova S. E., Mikheev E. V. Hetoceros: modulation of growth and composition under the action of salicylic acid // Agrarnaya Rossia. 2022. N. 10. P. 13–18.

5. Ljung K., Bhalerao R. P., Sandberg G. Sites and ho-meostatic control of auxin biosynthesis in Arabidopsis during vegetative growth // Plant Journal. 2001. V. 28. P. 465–474. DOI: 10.1046/j.1365-313x.2001.01173.x., Ljung K., Bhalerao R. P., Sandberg G. Sites and ho-meostatic control of auxin biosynthesis in Arabidopsis during vegetative growth // Plant Journal. 2001. V. 28. P. 465–474. DOI: 10.1046/j.1365-313x.2001.01173.x.