The Effect of Nisin and Chloramphenicol Combinationon Staphylococcus aureus ATCC 6538 Biofilm Structure-Reference-Cited by-同舟云学术

The Effect of Nisin and Chloramphenicol Combinationon Staphylococcus aureus ATCC 6538 Biofilm Structure

Published:2022-12-31 Issue: Volume: Page:
ISSN:2458-7575
Container-title:Bilecik Şeyh Edebali Üniversitesi Fen Bilimleri Dergisi
language:tr
Short-container-title:

Author:

KOÇAK Umut Çağrı¹,ARSLAN Elif¹,ÇOBANOĞLU Şeymanur¹,COŞKUN Muhammed Kürşad¹,YAZICI Ayşenur²^ORCID,ÖRTUCU Serkan¹

Affiliation:

1. ERZURUM TECHNICAL UNIVERSITY

2. Erzurum Teknik Üniversitesi, Yüksek Teknoloji ve Araştırma Merkezi (YUTAM)

Abstract

Nowadays, due to the rapid spread of antibiotic resistance and the difficulty of discovering new antimicrobial agents, the reuse and combinational strategies of old antibiotics have come to the fore. In this study, we investigated the combinational efficacy of nisin and chloramphenicol against the ATCC 6538 strain of Staphylococcus aureus. The MIC values of nisin and chloramphenicol were >64 and 32 µg/mL, respectively. The checkerboard assay was carried out for the inspection of synergism between nisin and chloramphenicol. Furthermore, the crystal violet assay was employed to assess antibiofilm effects. Additionally, the expressions of various virulence genes (agrA, spa, icaA and saeR) were investigated using the colony biofilm assay and qRT-PCR methods. In combination application, MIC values of nisin and chloramphenicol was decreased. In addition, biofilm formation was decreased.It was shown that the expression level of the agrA gene decreased compared to the control in the nisin, chloramphenicol and combinational applications. The expression levels of other genes were increased compared to the control. The results showed that the activities of nisin and chloramphenicol combinations had synergistic and antibiofilm activity. This study sheds light on the combinatorial use of older antibiotics.

Publisher

Bilecik Seyh Edebali Universitesi Fen Bilimleri Dergisi

Subject

General Earth and Planetary Sciences,General Environmental Science

Reference32 articles.

1. Cascioferro, S., Carbone, D., Parrino, B., Pecoraro, C., Giovannetti, E., Cirrincione, G., & Diana, P. (2021). Therapeutic Strategies to Counteract Antibiotic Resistance in MRSA Biofilm‐Associated Infections. Chemmedchem, 16(1), 65-80.

2. Kakoullis, L., Papachristodoulou, E., Chra, P., & Panos, G. (2021). Mechanisms of Antibiotic Resistance In Important Gram-Positive And Gram-Negative Pathogens And Novel Antibiotic Solutions. Antibiotics, 10(4), 415.

3. Seethalakshmi, P. S., Rajeev, R., Kiran, G. S., & Selvin, J. (2020). Promising Treatment Strategies To Combat Staphylococcus Aureus Biofilm Infections: An Updated Review. Biofouling, 36(10), 1159-1181.

4. Worthington, R. J., & Melander, C. (2013). Combination Approaches To Combat Multidrug-Resistant Bacteria. Trends In Biotechnology, 31(3), 177-184.

5. Angst, D. C., Tepekule, B., Sun, L., Bogos, B., & Bonhoeffer, S. (2021). Comparing Treatment Strategies To Reduce Antibiotic Resistance In An In Vitro Epidemiological Setting. Proceedings of the National Academy of Sciences, 118(13), E2023467118.