Prophylactic impact of nano-selenium on performance, carcasses quality, and tissues' selenium concentration using reversed-phase high-performance liquid chromatography during microbial challenge in broiler chickens
-
Published:2020
Issue:9
Volume:13
Page:1780-1797
-
ISSN:2231-0916
-
Container-title:Veterinary World
-
language:en
-
Short-container-title:Vet World
Author:
Soliman Essam S.1ORCID, Mahmoud Fadwa F.2ORCID, Fadel Mai A.3ORCID, Hamad Rania T.4ORCID
Affiliation:
1. Department of Animal Hygiene, Zoonosis and Animal Behavior, Faculty of Veterinary Medicine, Suez Canal University, Ismailia 41522, Egypt. 2. Food Hygiene and Microbiology, Reference Laboratory for Veterinary Quality Control on Poultry Production, Animal Health Research Institute, Agriculture Research Center, Ismailia 41511, Egypt. 3. Pharmacology and Pyrogen Unit, Chemistry and Food Deficiency Department, Animal Health Research Institute, Agriculture Research Center, Dokki, Giza 12618, Egypt. 4. Department of Pathology, Faculty of Veterinary Medicine, Menoufia University, Al Minufya 33511, Egypt.
Abstract
Background and Aim: Nano-selenium (NS) supplementation contributes in improving productivity, performance, and meat quality while reducing public health concern. Influence of NS and inorganic selenium (Se) water additive on performance, carcass quality, immunoglobulin concentration, intestinal microbiota, Se tissue concentrations, and tissue architecture was studied.
Materials and Methods: Two-hundred and sixty 1-day-old Hubbard chicks were randomly grouped into five groups (5×52) and supplemented with 0.5 and 1.0 mL of NS and inorganic Se (100 mg.L-1). G1, G2, G3, and G4 were challenged with Escherichia coli O157: H7 2.6×108 on the 14th day. A total of 2250 samples, including 250 sera, 250 intestinal swabs, and 1500 organ and tissue samples as liver, spleen, heart, bursa, intestine, and breast muscles, and 250 eviscerated carcasses were collected.
Results: The results revealed a highly significant increase (p<0.01) in live body weights, weight gains, performance indices, carcasses, and organs weights, whereas immunoglobulin G and M concentrations in broilers treated with 0.5 and 1.0 mL NS, respectively, synchronized reveal a highly significant decline (p<0.01) in total bacterial and Enterobacteriaceae counts of intestinal swabs and breast muscles, final pH24, and drip loss in broilers treated with 0.5 and 1.0 mL NS, respectively. Meanwhile, water holding capacity revealed no significant differences between all groups. Reversed-phase high-performance liquid chromatography examination revealed the earlier disappearance of NS residues than inorganic Se from the broiler's liver and muscles. Histopathological photomicrographs of the liver, spleen, bursa of Fabricius, and intestine, as well as, the immunohistochemistry of intestinal sections revealed superior tissue architecture in broilers treated with NS contrary to inorganic Se.
Conclusion: The study showed significant stimulation actions of NS on performance, immunity, carcass and meat quality, intestinal and muscles' bacterial load as well as short withdrawal period and nearly normal cellular architecture compared to inorganic Se.
Publisher
Veterinary World
Subject
General Veterinary
Reference83 articles.
1. Yaqoop, P. (2017) Impact of probiotics on the immune response to influenza vaccination is strongly influenced by ageing. Nutr. Bull., 42(1): 87-93. 2. Plaza-Diaz, J., Ruiz-Ojeda, F.J., Gil-Capos, M. and Gil, A. (2018) Immune-mediated mechanisms of action of probiotics and synbiotics in treating pediatric intestinal diseases. Nutrients, 10(1): 42. 3. Soliman, E.S., Hamad, R.T. and Ahmed, A. (2017) Prophylactic and immune-modulatory influences of Nigella sativa Linn. In broilers exposed to biological challenge. Vet. World, 10(12): 1447-1455. 4. Yang, X., Long, F., Xin, H., Yang, C. and Yang, X. (2018) Impact of essential oils and organic acids on the growth performance, digestive functions and immunity of broiler chickens. Anim. Nutr., 4(4): 388-393. 5. Pan, T., Liu, T., Tan, S., Wan, N., Zhang, Y. and Li, S. (2018) Lower selenoprotein T expression and immune response in the immune organs of broilers with exudative diathesis due to selenium deficiency. Biol. Trace Elem. Res., 182(2): 364-372.
Cited by
8 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献
|
|