Antioxidant influence on poultry liver morphology and hepatocyte ultrastructure

Abstract

Background and Aim: The poultry farming development is held back by necessity to use the concentrates with the increased number of crude protein, mycotoxicoses, and subclinical infections concentration. They make a significant impact on the liver, therefore affecting its morphofunctional condition. Antioxidants use can prevent the negative influence of these factors. This study aimed to examine the impact of feed supplements containing natural antioxidants and synthetic antioxidants. Materials and Methods: The Muscovy ducks, Hungarian White geese, and quails were the study object. Birds after hatching from eggs were split into two groups: Control and two experimental. The control group (40 birds of each species) received a normal diet in accordance with the type and age. The young ducklings, goslings, and quails of the first experimental group (30 birds of each species) received water with diisopropylammonium dichloroacetate (Dironax). The young ducklings, goslings, and quails of the second experimental group (30 birds of each species) received liquid multivitamin preparation, containing organic selenium form (Solvimin Selen) from the 1st day of the postembryonic development to the age of 60 days. We performed the weighing of the young ducklings, goslings, and quails, determined the live weight, liver weight, using the electronic scales (measurement inaccuracy is 0.02 g). To conduct the morphometric, histological, and electron microscopic studies liver, we killed the birds at the age of 1 day, 15 days, a month and 2 months during the postembryonic ontogenesis. Results: The performed overall studies allowed to determine the positive influence of the antioxidants on growth and development of the meat bird, whose body mass increased by 5-10% in comparison with the control parameters. The antioxidants use prevents the development of fatty, hydropic and parenchymal degeneration, hepatocyte and epithelial cells necrosis of the bile ducts, and connective tissue proliferation with its further fibrosis. Conclusion: This study proved that it is more effective to use well-digestible, fast-acting natural polyvitaminic antioxidant complex with selenium, starting from the 1st day of the postembryonic ontogeny.