Phenotypic and genotypic resistance to antibiotics in Staphylococcus aureus strains isolated from cattle milk in Northern Kazakhstan
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Published:2023-09
Issue:
Volume:
Page:1815-1820
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ISSN:2231-0916
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Container-title:Veterinary World
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language:en
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Short-container-title:Vet World
Author:
Baymenov Bakhit Muratovich1ORCID, Bulashev Aitbay Kabykeshovich2ORCID, Сhuzhebayeva Gulzhagan Dzhambulovna1ORCID, Aliyeva Gulnur Kozyevna1ORCID, Beishova Indira Saltanovna3ORCID, Kokanov Sabit Kabdyshevich1, Raketsky Vitaly Anatolevich4ORCID
Affiliation:
1. Research Institute of Applied Biotechnology, A. Baitursynov Kostanay Regional University, Kostanai, Republic of Kazakhstan. 2. Department of Microbiology and Biotechnology, S. Seifullin Kazakh Agro Technical Research University, Astana, Republic of Kazakhstan. 3. Testing Center, West Kazakhstan Agrarian and Technical University Named after Zhangir Khan, Uralsk, Republic of Kazakhstan. 4. Department of Veterinary Medicine, A. Baitursynov Kostanay Regional University, Kostanai, Republic of Kazakhstan.
Abstract
Background and Aim: Staphylococcus aureus is the most frequent and ubiquitous cause of mastitis in cows. In recent decades, antibiotic resistance has rapidly spread among infectious disease pathogens in Kazakhstan and globally. This study examined the phenotypic and genotypic resistance of S. aureus strains obtained from cattle milk to antibiotics.
Materials and Methods: In 2021 and 2022, 675 cow milk samples were collected from 16 dairy farms in Northern Kazakhstan. Staphylococcus aureus was identified using culture and biochemical methods. The nature of antibiotic resistance was determined by the disk diffusion (DD) method. The distribution of antibiotic resistance genes was determined by polymerase chain reaction.
Results: Among the obtained S. aureus isolates, high levels of resistance to β-lactam antibiotics (100%), tetracyclines (95.4%), fluoroquinolones (95.4%), and macrolides (60.92%) were observed. Meanwhile, the lowest levels of resistance were identified for sulfonamides (21.84%) and aminoglycosides (27.59%). All the obtained isolates were positive for the nuc gene encoding thermonuclease. The blaZ, ermC, and tetK genes were detected in 45.9%, 77%, and 83.9% of the studied S. aureus isolates, respectively.
Conclusion: The results indicate a high prevalence of antibiotic resistance in S. aureus isolated from cows with clinical and subclinical forms of mastitis in Northern Kazakhstan. In addition, the prevalence of resistance was higher when evaluated by the DD method than when detecting the specific antibiotic resistance genes blaZ, tetK, and ermC, indicating the need for deeper analysis of the phenotypic and genetic determinants of antibiotic resistance.
Keywords: antibiotic resistance genes, cow milk, polymerase chain reaction, Staphylococcus aureus strains.
Funder
Ministry of Agriculture of the Republic of Kazakhstan
Publisher
Veterinary World
Subject
General Veterinary
Reference30 articles.
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2 articles.
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