Ischemia-Induced Metabolic Patterns Associate With Kidney Function During Normothermic Kidney Perfusion, a Preclinical Study

Author:

De Beule Julie12,De Craemer Sam2,Verstraeten Laurence12,Ghesquière Bart23,Jochmans Ina14

Affiliation:

1. Department of Microbiology, Immunology, and Transplantation, Laboratory of Abdominal Transplantation, Transplantation Research Group, KU Leuven, 3000 Leuven, Belgium

2. Center for Cancer Biology, Metabolomics Core Facility Leuven, VIB, 3000 Leuven, Belgium

3. Department of Cellular and Molecular Medicine, Laboratory of Applied Mass Spectrometry, KU Leuven, 3000 Leuven, Belgium

4. Department of Abdominal Transplant Surgery, University Hospitals Leuven, 3000 Leuven, Belgium

Abstract

Objective: To investigate if ischemia alters donor kidney metabolism and whether these changes associate with organ function. Summary Background Data: An unmet need in kidney transplantation is the ability to predict post-transplant organ function before transplantation. Key to such viability testing is a profound understanding of the organ’s complex biochemistry and how ischemia, inevitable during the transplantation process, influences this. Methods: First, metabolic changes in glucose, lactate and 20 amino acids induced by no, 1h of warm, or 22h of cold ischemia were investigated during 4h perfusion of pig kidneys with autologous whole blood (n=6/group), simulating the ischemia-reperfusion phase of transplantation. Next, we confirmed similar metabolic changes during normothermic preservation of pig (n=3/group; n=4 for cold ischemia) and discarded human kidneys (n=6) perfused with a red-blood cell based perfusate. Results: At 2h of perfusion with autologous whole blood, abundances of 17/20 amino acids were significantly different between groups, reflecting the type of ischemia. Amino acid changes at 15 min and 2h of perfusion correlated with future kidney function during perfusion. Similar metabolic patterns were observed during perfusion preservation of pig and discarded human donor kidneys, suggesting an opportunity to assess kidney viability before transplantation. Conclusions: Perfusate metabolite changes during normothermic kidney perfusion represent a unique non-invasive opportunity to assess graft viability. These findings now need validation in transplant studies.

Publisher

Ovid Technologies (Wolters Kluwer Health)

Subject

Surgery

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3