Gut-licensed β7 + CD4 + T cells contribute to progressive retinal ganglion cell damage in glaucoma

Author:

He Chong12ORCID,Xiu Wenbo1ORCID,Chen Qinyuan1ORCID,Peng Kun3ORCID,Zhu Xiong14ORCID,Wang Zuo15ORCID,Xu Xiang1ORCID,Chen Yang1ORCID,Zhang Gao1ORCID,Fu Jing1ORCID,Dong Qiwei1ORCID,Wu Xiaoqiong6ORCID,Li An1ORCID,Liu Donghua3ORCID,Gao Yanping1ORCID,Wang Jinxia1ORCID,Wang Zhao27ORCID,Deng Bolin8ORCID,Shuai Ping3ORCID,Gao Caiping9ORCID,Chen Yilian8ORCID,Yu Ling10ORCID,Lu Fang123ORCID

Affiliation:

1. Clinical Immunology Translational Medicine Key Laboratory of Sichuan Province, Sichuan Provincial People’s Hospital, University of Electronic Science and Technology of China, Chengdu, China.

2. Medico-Engineering Cooperation on Applied Medicine Research Center, University of Electronic Science and Technology of China, Chengdu, China.

3. Health Management Center, Sichuan Provincial People’s Hospital, University of Electronic Science and Technology of China, Chengdu, China.

4. Department of Prenatal Diagnosis, Chengdu Women's and Children’s Central Hospital, School of Medicine, University of Electronic Science and Technology of China, Chengdu, China.

5. Department of Clinical Laboratory, Sichuan Cancer Hospital and Institute, Sichuan Cancer Center, School of Medicine, University of Electronic Science and Technology of China, Chengdu, China.

6. Department of Ophthalmology, Luzhou Meternal and Child Health Hospital, Luzhou, China.

7. School of Electronic Science and Engineering, University of Electronic Science and Technology of China, Chengdu, China.

8. Department of Ophthalmology, Sichuan Provincial People’s Hospital, University of Electronic Science and Technology of China, Chengdu, China.

9. Department of Gastroenterology, Sichuan Provincial People’s Hospital, University of Electronic Science and Technology of China, Chengdu, China.

10. Department of Ophthalmology, Daping Hospital, Army Medical Center, Army Medical University, Chongqing, China.

Abstract

Glaucoma is the leading cause of irreversible blindness. Currently, most therapeutic strategies aim to reduce elevated intraocular pressure (EIOP), but this does not always halt disease progression. Evidence suggests a role for T cells in glaucoma pathogenesis, but the underlying mechanisms remain largely unknown. Here, we found that the percentage of circulating CD4 + T cells expressing a gut-homing integrin β7 was increased in patients with glaucoma and was associated with disease stage. In an EIOP-triggered glaucoma mouse model, β7 + CD4 + T cells infiltrated the retina in the progressive phase of glaucoma via eliciting retinal endothelial cell expression of mucosal vascular addressin cell adhesion molecule 1 (MAdCAM-1). MAdCAM-1 was minimally detected in retinas of healthy mice, and neutralization with an MAdCAM-1 antibody ameliorated retinal ganglion cell (RGC) loss and glial activity in mice with glaucoma. We furthermore found that EIOP-induced β7 + CD4 + T cells homed to the gut during the acute phase of glaucoma, which was essential for progressive RGC damage in diseased mice. Gut-homing β7 + CD4 + T cells underwent transcriptional reprogramming, showing up-regulated pathways enriched in autoimmune diseases, bacteria responses, mucosal immunity, and glial activity. Gut-homing β7 + CD4 + T cells gained the competence to induce retinal MAdCAM-1 expression and to cross the blood-retina barrier. Together, our study reveals a role of gut-licensed β7 + CD4 + T cells and MAdCAM-1 in RGC degeneration and emphasizes the importance of the “gut-retina” axis in glaucoma.

Publisher

American Association for the Advancement of Science (AAAS)

Subject

General Medicine

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