Simultaneous electromechanical monitoring in engineered heart tissues using a mesoscale framework-Reference-Cited by-同舟云学术

Simultaneous electromechanical monitoring in engineered heart tissues using a mesoscale framework

Published:2024-09-13 Issue:37 Volume:10 Page:
ISSN:2375-2548
Container-title:Science Advances
language:en
Short-container-title:Sci. Adv.

Author:

Fullenkamp Dominic E.¹^ORCID,Maeng Woo-Youl²³^ORCID,Oh Seyong⁴^ORCID,Luan Haiwen²³⁵^ORCID,Kim Kyung Su²³⁶⁷^ORCID,Chychula Ivana A.¹^ORCID,Kim Jin-Tae²³⁸^ORCID,Yoo Jae-Young⁹^ORCID,Holgren Cory W.¹,Demonbreun Alexis R.¹^ORCID,George Sharon¹⁰¹¹^ORCID,Li Binjie¹⁰^ORCID,Hsu Yaching²³¹⁰,Chung Gooyoon¹²^ORCID,Yoo Jeongmin¹²^ORCID,Koo Jahyun⁶⁷^ORCID,Park Yoonseok¹²¹³^ORCID,Efimov Igor R.¹⁰¹⁴^ORCID,McNally Elizabeth M.¹^ORCID,Rogers John A.²³¹⁰¹⁵¹⁶^ORCID

Affiliation:

1. Center for Genetic Medicine, Bluhm Cardiovascular Institute, Northwestern University Feinberg School of Medicine, Chicago, IL 60611, USA.

2. Querrey Simpson Institute for Bioelectronics, Northwestern University, Evanston, IL 60208, USA.

3. Center for Bio-Integrated Electronics, Northwestern University, Evanston, IL 60208, USA.

4. Division of Electrical Engineering, Hanyang University ERICA, Ansan 15588, Republic of Korea.

5. Department of Mechanical and Aerospace Engineering, University of California, San Diego, La Jolla, CA 92093, USA.

6. School of Biomedical Engineering, Korea University, Seoul 02841, Republic of Korea.

7. Interdisciplinary Program in Precision Public Health, Korea University, Seoul 02841, Republic of Korea.

8. Department of Mechanical Engineering, Pohang University of Science and Technology, Pohang 37673, Republic of Korea.

9. Department of Semiconductor Convergence Engineering, Sungkyunkwan University, Suwon 16417, Republic of Korea.

10. Department of Biomedical Engineering, Northwestern University, Evanston, IL 60208, USA.

11. Department of Pharmacology and Chemical Biology, University of Pittsburgh, Pittsburgh, PA 15260, USA.

12. Department of Advanced Materials Engineering for Information and Electronics, Integrated Education Institute for Frontier Science & Technology (BK21 Four), Kyung Hee University, Yongin 17104, Republic of Korea.

13. KHU-KIST Department of Converging Science and Technology, Kyung Hee University, Seoul 02447, Republic of Korea.

14. Department of Medicine, Northwestern University, Chicago, IL 60611, USA.

15. Department of Materials Science and Engineering, Northwestern University, Evanston, IL 60208, USA.

16. Department of Neurological Surgery, Northwestern University, Chicago, IL 60611, USA.

Abstract

Engineered heart tissues (EHTs) generated from human induced pluripotent stem cell–derived cardiomyocytes (hiPSC-CMs) represent powerful platforms for human cardiac research, especially in drug testing and disease modeling. Here, we report a flexible, three-dimensional electronic framework that enables real-time, spatiotemporal analysis of electrophysiologic and mechanical signals in EHTs under physiological loading conditions for dynamic, noninvasive, longer-term assessments. These electromechanically monitored EHTs support multisite measurements throughout the tissue under baseline conditions and in response to stimuli. Demonstrations include uses in tracking physiological responses to pharmacologically active agents and in capturing electrophysiological characteristics of reentrant arrhythmias. This platform facilitates precise analysis of signal location and conduction velocity in human cardiomyocyte tissues, as the basis for a broad range of advanced cardiovascular studies.

Publisher

American Association for the Advancement of Science (AAAS)

Link

https://www.science.org/doi/pdf/10.1126/sciadv.ado7089

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