Affiliation:
1. Beijing Advanced Innovation Center for Structural Biology, Tsinghua-Peking Joint Center for Life Sciences, School of Life Sciences, Tsinghua University, Beijing 100084, China.
Abstract
How spliceosomes make the first cut
In eukaryotes, transcribed precursor mRNA includes noncoding sequences that must be spliced out. This is done by the spliceosome, a dynamic complex in which five small nuclear RNAs and several proteins go through a series of ordered interactions and conformational rearrangements to achieve splicing. Two protein structures provide a look at the first catalytic step in the pathway. Yan
et al.
report the structure of the activated spliceosome (the B
act
complex) at 3.5 Å resolution, revealing how latency is maintained even though the complex is mostly primed for catalysis. Wan
et al.
report the structure of the catalytic step 1 spliceosome (the C complex) at 3.4 Å resolution; this complex forms after the first step of the splicing reaction.
Science
, this issue pp.
904
and
895
Funder
Ministry of Science and Technology
National Natural Science Foundation of China
Publisher
American Association for the Advancement of Science (AAAS)
Reference93 articles.
1. RNA catalyses nuclear pre-mRNA splicing
2. M. J. Moore C. C. Query P. A. Sharp “Splicing of precursors to mRNA by the spliceosome.”in The RNA World R. F. Gesteland J. F. Atkins Eds. (Cold Spring Harbor Monograph Archive Cold Spring Harbor Laboratory Press 1993) pp. 303–357.
3. The Spliceosome: Design Principles of a Dynamic RNP Machine
4. Spliceosome Structure and Function
5. The mechanism of the second step of pre-mRNA splicing
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