Population transcriptomics of human malaria parasites reveals the mechanism of artemisinin resistance-Reference-Cited by-同舟云学术

Population transcriptomics of human malaria parasites reveals the mechanism of artemisinin resistance

Published:2015-01-23 Issue:6220 Volume:347 Page:431-435
ISSN:0036-8075
Container-title:Science
language:en
Short-container-title:Science

Author:

Mok Sachel¹,Ashley Elizabeth A.²³,Ferreira Pedro E.¹,Zhu Lei¹,Lin Zhaoting¹,Yeo Tomas¹,Chotivanich Kesinee⁴,Imwong Mallika⁵,Pukrittayakamee Sasithon⁴,Dhorda Mehul³⁶⁷,Nguon Chea⁸,Lim Pharath⁸⁹,Amaratunga Chanaki⁹,Suon Seila⁸,Hien Tran Tinh¹⁰,Htut Ye¹¹,Faiz M. Abul¹²,Onyamboko Marie A.¹³,Mayxay Mayfong¹⁴¹⁵,Newton Paul N.²³¹⁴,Tripura Rupam²,Woodrow Charles J.²³,Miotto Olivo²¹⁶¹⁷,Kwiatkowski Dominic P.¹⁶¹⁷,Nosten François³¹⁸,Day Nicholas P. J.²³,Preiser Peter R.¹,White Nicholas J.²³,Dondorp Arjen M.²³,Fairhurst Rick M.⁹,Bozdech Zbynek¹

Affiliation:

1. School of Biological Sciences, Nanyang Technological University, Singapore.

2. Mahidol-Oxford Tropical Medicine Research Unit, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand.

3. Centre for Tropical Medicine and Global Health, Nuffield Department of Medicine, University of Oxford, Oxford, UK.

4. Department of Clinical Tropical Medicine, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand.

5. Department of Molecular Tropical Medicine and Genetics, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand.

6. WorldWide Antimalarial Resistance Network (WWARN), Asia Regional Centre, Mahidol University, Bangkok, Thailand.

7. WorldWide Antimalarial Resistance Network, University of Maryland School of Medicine, Baltimore, MD, USA.

8. National Center for Parasitology, Entomology and Malaria Control, Phnom Penh, Cambodia.

9. Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, USA.

10. Oxford University Clinical Research Unit (OUCRU), Hospital for Tropical Diseases, Ho Chi Minh City, Vietnam.

11. Department of Medical Research, Lower Myanmar, Yangon, Myanmar.

12. Malaria Research Group & Dev Care Foundation, Dhaka, Bangladesh.

13. Kinshasa School of Public Health, Kinshasa, Democratic Republic of the Congo.

14. Lao–Oxford–Mahosot Hospital–Wellcome Trust Research Unit, Mahosot Hospital, Vientiane, Lao PDR.

15. Faculty of Postgraduate Studies, University of Health Sciences, Vientiane, Lao PDR.

16. Medical Research Council (MRC) Centre for Genomics and Global Health, University of Oxford, Oxford, UK.

17. Wellcome Trust Sanger Institute, Hinxton, Cambridge, UK.

18. Shoklo Malaria Research Unit, Mahidol-Oxford Tropical Medicine Research Unit, Faculty of Tropical Medicine, Mahidol University, Mae Sot, Thailand.

Abstract

Mechanisms propelling drug resistance If it were to spread, resistance to the drug artemisinin would seriously derail the recent gains of global malaria control programs (see the Perspective by Sibley). Mutations in a region called the K13-propeller are predictive for artemisinin resistance in Southeast Asia. Mok et al. looked at the patterns of gene expression in parasites isolated from more than 1000 patients sampled in Africa, Bangladesh, and the Mekong region. A range of mutations that alter protein repair pathways and the timing of the parasite's developmental cycle were only found in parasites from the Mekong region. Straimer et al. genetically engineered the K13 region of parasites obtained from recent clinical isolates. Mutations in this region were indeed responsible for the resistance phenotypes. Science , this issue p. 431 , p. 428 ; see also p. 373

Funder

National Institute of Allergy and Infectious Diseases (NIAID)

Bill & Melinda Gates Foundation

Singapore National Medical Council

Wellcome Trust Sanger Institute

Publisher

American Association for the Advancement of Science (AAAS)

Subject

Multidisciplinary

Reference42 articles.

1. Artemisinin Resistance inPlasmodium falciparumMalaria

2. Artemisinin-resistant Plasmodium falciparum in Pursat province, western Cambodia: a parasite clearance rate study