A naturally isolated symbiotic bacterium suppresses flavivirus transmission by <i>Aedes</i> mosquitoes-Reference-Cited by-同舟云学术

A naturally isolated symbiotic bacterium suppresses flavivirus transmission by Aedes mosquitoes

Published:2024-04-19 Issue:6693 Volume:384 Page:
ISSN:0036-8075
Container-title:Science
language:en
Short-container-title:Science

Author:

Zhang Liming¹²³^ORCID,Wang Daxi⁴^ORCID,Shi Peibo⁴⁵^ORCID,Li Juzhen¹,Niu Jichen¹,Chen Jielong¹,Wang Gang¹,Wu Linjuan¹,Chen Lu¹^ORCID,Yang Zhenxing⁶,Li Susheng⁶,Meng Jinxin⁶,Ruan Fangchao⁷,He Yuwen⁶,Zhao Hailong⁴^ORCID,Ren Zirui⁴^ORCID,Wang Yibaina⁸,Liu Yang²,Shi Xiaolu³,Wang Yunfu⁹,Liu Qiyong¹⁰^ORCID,Li Junhua⁴^ORCID,Wang Penghua¹¹^ORCID,Wang Jinglin⁶^ORCID,Zhu Yibin¹³^ORCID,Cheng Gong¹²³¹²^ORCID

Affiliation:

1. New Cornerstone Science Laboratory, Tsinghua University-Peking University Joint Center for Life Sciences, School of Basic Medical Sciences, Tsinghua University, Beijing 100084, China.

2. Institute of Infectious Diseases, Shenzhen Bay Laboratory, Shenzhen 518000, China.

3. Institute of Pathogenic Organisms, Shenzhen Center for Disease Control and Prevention, Shenzhen 518055, China.

4. Shenzhen Key Laboratory of Unknown Pathogen Identification, BGI Research, Shenzhen 518083, China.

5. College of Life Sciences, University of Chinese Academy of Sciences, Beijing 100049, China.

6. Yunnan Tropical and Subtropical Animal Viral Disease Laboratory, Yunnan Animal Science and Veterinary Institute, Kunming, Yunnan 650000, China.

7. Kunming Medical University, Kunming, Yunnan 650000, China.

8. China National Center for Food Safety Risk Assessment, Beijing 100022, China.

9. Institute of Neuroscience, Hubei University of Medicine, Shiyan, Hubei 442000, China.

10. National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China.

11. Department of Immunology, School of Medicine, University of Connecticut Health Center, Farmington, CT 06030, USA.

12. Southwest United Graduate School, Kunming 650092, China.

Abstract

The commensal microbiota of the mosquito gut plays a complex role in determining the vector competence for arboviruses. In this study, we identified a bacterium from the gut of field Aedes albopictus mosquitoes named Rosenbergiella sp. YN46 ( Rosenbergiella_ YN46) that rendered mosquitoes refractory to infection with dengue and Zika viruses. Inoculation of 1.6 × 10 3 colony forming units (CFUs) of Rosenbergiella_ YN46 into A. albopictus mosquitoes effectively prevents viral infection. Mechanistically, this bacterium secretes glucose dehydrogenase ( Ry GDH), which acidifies the gut lumen of fed mosquitoes, causing irreversible conformational changes in the flavivirus envelope protein that prevent viral entry into cells. In semifield conditions, Rosenbergiella_ YN46 exhibits effective transstadial transmission in field mosquitoes, which blocks transmission of dengue virus by newly emerged adult mosquitoes. The prevalence of Rosenbergiella_ YN46 is greater in mosquitoes from low-dengue areas (52.9 to ~91.7%) than in those from dengue-endemic regions (0 to ~6.7%). Rosenbergiella_ YN46 may offer an effective and safe lead for flavivirus biocontrol.

Publisher

American Association for the Advancement of Science (AAAS)

Link

https://www.science.org/doi/pdf/10.1126/science.adn9524

Reference73 articles.

1. The continued threat of emerging flaviviruses

2. The global distribution and burden of dengue

3. The emergence of Zika virus and its new clinical syndromes

4. Progress towards Understanding the Mosquito-Borne Virus Life Cycle

5. Progress towards research on mosquito-borne arboviral transmission and infection

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