SARS-CoV-2 infection protects against rechallenge in rhesus macaques-Reference-Cited by-同舟云学术

SARS-CoV-2 infection protects against rechallenge in rhesus macaques

Published:2020-05-20 Issue:6505 Volume:369 Page:812-817
ISSN:0036-8075
Container-title:Science
language:en
Short-container-title:Science

Author:

Chandrashekar Abishek¹^ORCID,Liu Jinyan¹^ORCID,Martinot Amanda J.¹²^ORCID,McMahan Katherine¹^ORCID,Mercado Noe B.¹^ORCID,Peter Lauren¹^ORCID,Tostanoski Lisa H.¹^ORCID,Yu Jingyou¹^ORCID,Maliga Zoltan³^ORCID,Nekorchuk Michael⁴,Busman-Sahay Kathleen⁴^ORCID,Terry Margaret⁴^ORCID,Wrijil Linda M.²^ORCID,Ducat Sarah²^ORCID,Martinez David R.⁵,Atyeo Caroline³⁶^ORCID,Fischinger Stephanie⁶^ORCID,Burke John S.⁶^ORCID,Slein Matthew D.⁶^ORCID,Pessaint Laurent⁷^ORCID,Van Ry Alex⁷^ORCID,Greenhouse Jack⁷^ORCID,Taylor Tammy⁷^ORCID,Blade Kelvin⁷^ORCID,Cook Anthony⁷,Finneyfrock Brad⁷^ORCID,Brown Renita⁷,Teow Elyse⁷,Velasco Jason⁷,Zahn Roland⁸^ORCID,Wegmann Frank⁸^ORCID,Abbink Peter¹^ORCID,Bondzie Esther A.¹^ORCID,Dagotto Gabriel¹³^ORCID,Gebre Makda S.¹³^ORCID,He Xuan¹,Jacob-Dolan Catherine¹³^ORCID,Kordana Nicole¹^ORCID,Li Zhenfeng¹,Lifton Michelle A.¹^ORCID,Mahrokhian Shant H.¹^ORCID,Maxfield Lori F.¹^ORCID,Nityanandam Ramya¹^ORCID,Nkolola Joseph P.¹^ORCID,Schmidt Aaron G.⁶⁹^ORCID,Miller Andrew D.¹⁰^ORCID,Baric Ralph S.⁵^ORCID,Alter Galit⁶⁹^ORCID,Sorger Peter K.³^ORCID,Estes Jacob D.⁴^ORCID,Andersen Hanne⁷^ORCID,Lewis Mark G.⁷^ORCID,Barouch Dan H.¹⁶⁹^ORCID

Affiliation:

1. Center for Virology and Vaccine Research, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02215, USA.

2. Tufts University Cummings School of Veterinary Medicine, North Grafton, MA 01536, USA.

3. Harvard Medical School, Boston, MA 02115, USA.

4. Oregon Health & Sciences University, Beaverton, OR 97006, USA.

5. University of North Carolina, Chapel Hill, NC 27599, USA.

6. Ragon Institute of MGH, MIT, and Harvard, Cambridge, MA 02139, USA.

7. Bioqual, Rockville, MD 20852, USA.

8. Janssen Vaccines & Prevention BV, Leiden, Netherlands.

9. Massachusetts Consortium on Pathogen Readiness, Boston, MA 02215, USA.

10. Cornell University College of Veterinary Medicine, Ithaca, NY 14853, USA.

Abstract

An understanding of protective immunity to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is critical for vaccine and public health strategies aimed at ending the global coronavirus disease 2019 (COVID-19) pandemic. A key unanswered question is whether infection with SARS-CoV-2 results in protective immunity against reexposure. We developed a rhesus macaque model of SARS-CoV-2 infection and observed that macaques had high viral loads in the upper and lower respiratory tract, humoral and cellular immune responses, and pathologic evidence of viral pneumonia. After the initial viral clearance, animals were rechallenged with SARS-CoV-2 and showed 5 log10 reductions in median viral loads in bronchoalveolar lavage and nasal mucosa compared with after the primary infection. Anamnestic immune responses after rechallenge suggested that protection was mediated by immunologic control. These data show that SARS-CoV-2 infection induced protective immunity against reexposure in nonhuman primates.

Funder

National Institutes of Health

Bill and Melinda Gates Foundation

Janssen Research and Development

Beth Israel Deaconess Medical Center

Ragon Institute of MGH, MIT and Harvard

Mark and Lisa Schwartz Foundation

Massachusetts Consortium on Pathogen Readiness

Burroughs Wellcome Fund

Emergent Ventures, Mercatus Center

Publisher

American Association for the Advancement of Science (AAAS)