Comparative Genome Sequencing for Discovery of Novel Polymorphisms in Bacillus anthracis-Reference-Cited by-同舟云学术

Comparative Genome Sequencing for Discovery of Novel Polymorphisms in Bacillus anthracis

Published:2002-06-14 Issue:5575 Volume:296 Page:2028-2033
ISSN:0036-8075
Container-title:Science
language:en
Short-container-title:Science

Author:

Read Timothy D.¹,Salzberg Steven L.¹,Pop Mihai¹,Shumway Martin¹,Umayam Lowell¹,Jiang Lingxia¹,Holtzapple Erik¹,Busch Joseph D.²,Smith Kimothy L.²,Schupp James M.²,Solomon Daniel²,Keim Paul²,Fraser Claire M.¹

Affiliation:

1. The Institute for Genomic Research, 9712 Medical Center Drive, Rockville, MD 20850, USA.

2. Department of Biological Sciences, Northern Arizona University, Flagstaff, AZ 86011, USA.

Abstract

Comparison of the whole-genome sequence of Bacillus anthracis isolated from a victim of a recent bioterrorist anthrax attack with a reference reveals 60 new markers that include single nucleotide polymorphisms (SNPs), inserted or deleted sequences, and tandem repeats. Genome comparison detected four high-quality SNPs between the two sequenced B. anthracis chromosomes and seven differences among different preparations of the reference genome. These markers have been tested on a collection of anthrax isolates and were found to divide these samples into distinct families. These results demonstrate that genome-based analysis of microbial pathogens will provide a powerful new tool for investigation of infectious disease outbreaks.

Publisher

American Association for the Advancement of Science (AAAS)

Subject

Multidisciplinary

Reference23 articles.

1. Centers for Disease Control Morb. Mortal. Wkly. Rep. 50 877 (2001).

2. Genetic Diversity in the Protective Antigen Gene of Bacillus anthracis

3. Multiple-Locus Variable-Number Tandem Repeat Analysis Reveals Genetic Relationships within Bacillus anthracis

4. Centers for Disease Control Morb. Mortal. Wkly. Rep. 50 909 (2001).

5. The index strain was sent from Fort Detrick to Porton Down UK in or after 1982 and was cured of the pXO1 and pXO2 virulence plasmids (Fig. 1). Two preparations of genomic DNA were sent to TIGR. The first was prepared in a laboratory at the University of California Berkeley in 1998 (“Porton1”). The second was prepared at Porton Down in 2001 from a bacterium grown from the original frozen culture (“Porton2”). The reported history of storage of the bacteria used to create the cultures provides no obvious reasons for the seven intrastrain chromosomal SNPs observed (Table 1).

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