Visualization and analysis of gene expression in tissue sections by spatial transcriptomics-Reference-Cited by-同舟云学术

Visualization and analysis of gene expression in tissue sections by spatial transcriptomics

Published:2016-07 Issue:6294 Volume:353 Page:78-82
ISSN:0036-8075
Container-title:Science
language:en
Short-container-title:Science

Author:

Ståhl Patrik L.¹²,Salmén Fredrik²,Vickovic Sanja²,Lundmark Anna²³,Navarro José Fernández¹²,Magnusson Jens¹,Giacomello Stefania²,Asp Michaela²,Westholm Jakub O.⁴,Huss Mikael⁴,Mollbrink Annelie²,Linnarsson Sten⁵,Codeluppi Simone⁵⁶,Borg Åke⁷,Pontén Fredrik⁸,Costea Paul Igor²,Sahlén Pelin²,Mulder Jan⁹,Bergmann Olaf¹,Lundeberg Joakim²,Frisén Jonas¹

Affiliation:

1. Department of Cell and Molecular Biology, Karolinska Institute, SE-171 77 Stockholm, Sweden.

2. Science for Life Laboratory, Division of Gene Technology, KTH Royal Institute of Technology, SE-106 91 Stockholm, Sweden.

3. Department of Dental Medicine, Division of Periodontology, Karolinska Institute, SE-141 04 Huddinge, Sweden.

4. Science for Life Laboratory, Department of Biochemistry and Biophysics, Stockholm University, Box 1031, SE-171 21 Solna, Sweden.

5. Division of Molecular Neuroscience, Department of Medical Biochemistry and Biophysics, Karolinska Institute, SE-17177 Stockholm, Sweden.

6. Department of Physiology and Pharmacology, Karolinska Institute, SE-17177 Stockholm, Sweden.

7. Division of Oncology and Pathology, Department of Clinical Sciences Lund, Lund University, SE-223 81 Lund, Sweden.

8. Department of Immunology, Genetics and Pathology, Uppsala University, SE-751 85 Uppsala, Sweden.

9. Science for Life Laboratory, Department of Neuroscience, Karolinska Institute, SE-171 77 Stockholm, Sweden.

Abstract

Spatial structure of RNA expression RNA-seq and similar methods can record gene expression within and among cells. Current methods typically lose positional information and many require arduous single-cell isolation and sequencing. Ståhl et al. have developed a way of measuring the spatial distribution of transcripts by annealing fixed brain or cancer tissue samples directly to bar-coded reverse transcriptase primers, performing reverse transcription followed by sequencing and computational reconstruction, and they can do so for multiple genes. Science , this issue p. 78

Funder

Knut och Alice Wallenberg Foundation

Swedish Foundation for Strategic Research

Swedish Cancer Society

Karolinska Institute

Tobias Stiftelsen

Torsten Söderbergs Stiftelse

Ragnar Söderbergs Stiftelse

StratRegen

Åke Wiberg Foundation

Jeansson Foundations

Swedish Research Council