Crystal Structure of a Nucleocapsid-Like Nucleoprotein-RNA Complex of Respiratory Syncytial Virus-Reference-Cited by-同舟云学术

Crystal Structure of a Nucleocapsid-Like Nucleoprotein-RNA Complex of Respiratory Syncytial Virus

Published:2009-11-27 Issue:5957 Volume:326 Page:1279-1283
ISSN:0036-8075
Container-title:Science
language:en
Short-container-title:Science

Author:

Tawar Rajiv G.¹,Duquerroy Stéphane¹²,Vonrhein Clemens³,Varela Paloma F.¹,Damier-Piolle Laurence¹,Castagné Nathalie⁴,MacLellan Kirsty⁵,Bedouelle Hugues⁶,Bricogne Gérard³,Bhella David⁵,Eléouët Jean-François⁴,Rey Félix A.¹

Affiliation:

1. Institut Pasteur, Unité de Virologie Structurale, Département de Virologie and CNRS Unité de Recherche Associée (URA) 3015, 25 Rue du Dr Roux, 75724 Paris Cedex 15, France.

2. Université Paris-Sud, Faculté d’Orsay, 91405 Orsay Cedex, France.

3. Global Phasing, Sheraton House, Castle Park, Cambridge CB3 0AX, UK.

4. L’Institut National de la Recherche Agronomique, Unité de Virologie et Immunologie Moléculaires, Domaine du Vilvert, 78350 Jouy-en-Josas, France.

5. Medical Research Council Virology Unit, University of Glasgow, Church Street, Glasgow G11 5JR, UK.

6. Institut Pasteur, CNRS URA 3012, 25 Rue du Dr Roux, 75724 Paris Cedex 15, France.

Abstract

RSV in 3D Respiratory syncytial virus (RSV) causes pneumonia and bronchiolitis in infants. RSV is an RNA virus in which the genomic RNA forms part of a nuclease-resistant helical ribonucleoprotein complex. Tawar et al. (p. 1279 ) now use x-ray and electron microscopy data to model the structure of this nucleocapsid complex and show how it can template RNA synthesis. The crystal structure shows RNA wrapped around a decameric ring of nucleocapsid protein. Combining this structure with electron microscopy data gives a model that shows how polymerase might read out the RNA bases without disassembling the nucleocapsid helix.

Publisher

American Association for the Advancement of Science (AAAS)

Subject

Multidisciplinary

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