Cryo-EM Study of the Chromatin Fiber Reveals a Double Helix Twisted by Tetranucleosomal Units-Reference-Cited by-同舟云学术

Cryo-EM Study of the Chromatin Fiber Reveals a Double Helix Twisted by Tetranucleosomal Units

Published:2014-04-25 Issue:6182 Volume:344 Page:376-380
ISSN:0036-8075
Container-title:Science
language:en
Short-container-title:Science

Author:

Song Feng¹²,Chen Ping¹,Sun Dapeng¹²,Wang Mingzhu¹,Dong Liping¹²,Liang Dan¹²,Xu Rui-Ming¹,Zhu Ping¹,Li Guohong¹

Affiliation:

1. National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China.

2. University of Chinese Academy of Sciences, Beijing 100049, China.

Abstract

Double Helix, Doubled Chromatin consists of genomic DNA packaged onto nucleosomes—double donut-shaped complexes of histone proteins. Roughly 150 base pairs of DNA are wrapped around each nucleosome with variable lengths of linker DNA in-between. Using cryogenic electron microscopy, Song et al. (p. 376 ; see the Perspective by

Travers

) determined the 11 angstrom–resolution structure of a 12-nucleosome string of DNA. The segment forms a 30-nanometer fiber structure, which is itself double-helical, like the DNA it packages.

Publisher

American Association for the Advancement of Science (AAAS)

Subject

Multidisciplinary

Reference41 articles.

1. Hydrodynamic Studies on Defined Heterochromatin Fragments Support a 30-nm Fiber Having Six Nucleosomes per Turn

2. Higher-order structure of nucleosome oligomers from short-repeat chromatin.

3. Histone H1 is located in the interior of the chromatin 30-nm filament

4. Chromatin higher-order structure studied by neutron scattering and scanning transmission electron microscopy.

5. Structure of the 3000Å chromatin filament: X-ray diffraction from oriented samples

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